Supporting information for Danikovitch-Miagkova et al. (2003) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.0837136100
Supporting Text
RON and MET comprise a unique two-member family of receptor tyrosine kinases involved in human and mouse carcingenesis. Although MET has been shown to harbor oncogenic mutations in the kinase domain in hereditary and sporadic forms of papillary renal cancers (1), a similar mechanism has not been shown to operate in human lung adenocarcinomas (2). In contrast, a truncated cytoplasmic activated form of the mouse ortholog of Ron (known as Stk) was shown to mediate carcinogenesis by the Friend virus in mice (3, 4) and operate in human erythroleukemia (A.D.M., unpublished data) and small cell lung carcinoma (D. Angeloni, unpublished data). The natural ligand for RON is the macrophage-stimulating protein.
In our studies we also used highly transfectable MadinDarby canine kidney [that do not express RON or MSP (ref. 5 and Danilkovitch, et al., unpublished data)] and human embryonic kidney 293 cell lines to allow facile detection of protein interactions and modifications such as tyrosine phosphorylation
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