Abbreviated incubation times for human prions in mice expressing a chimeric mouse-human prion protein transgene -- Data Supplement - Supporting Figures -- Proceedings of the National Academy of Sciences

Supporting information for Korth et al. (2003) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.2627989100

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Fig. 5.
Mutation scheme of chimeric mouse–human PrP constructs. In the original MHu2M construct (1), human PrP (blue) was inserted between residues 96 and 167 of mouse PrP (white), mutating a total of nine amino acids (gray numbers). For the present study, the original MHu2M construct was mutated at the C-terminal site, the N-terminal site, or both sites of the human insert, resulting in six transgenic mouse lines on the Prnp^0/0 background: Tg(MHu2M,M165V)17030/Prnp^0/0, Tg(MHu2M,E167Q)18990/Prnp^0/0, Tg(MHu2M,M165V,E167Q)22372/Prnp^0/0, Tg(MHu2M,S96N,M165V,E167Q)34098/Prnp^0/0, Tg(MHu2M,S96N)28386/Prnp^0/0, and Tg(MHu2M,S96N,M108L,M111V)42/Prnp^0/0.

1. Telling, G. C., Scott, M., Hsiao, K. K., Foster, D., Yang, S.-L., Torchia, M., Sidle, K. C. L., Collinge, J., DeArmond, S. J. & Prusiner, S. B. (1994) Proc. Natl. Acad. Sci. USA 91, 9936–9940.