Abbreviated incubation times for human prions in mice expressing a chimeric mouse-human prion protein transgene -- Data Supplement - Supporting Figures -- Proceedings of the National Academy of Sciences

Supporting information for Korth et al. (2003) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.2627989100

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Fig. 7.
Neuropathology of Tg22372 mice inoculated with different cases of Creutzfeldt–Jakob disease (CJD). Neuropathology shown by hematoxylin and eosin staining (a and b); GFAP immunostaining (c and d); and PrP immunostaining (e and f). Tg22372 mice were inoculated with sCJD(MM1) (a, c, and e) or vCJD (b, d, and f) prions. For the sporadic CJD (sCJD) (MM1) case, the caudate nucleus is depicted at25´objective; for the variant CJD (vCJD) case, the subcallosal region is depicted at 40´objective. On first passage of sCJD(MM1) prions, minimal differences in the degree of vacuolation, the intensity of astrocytic gliosis, and PrP immunostaining after hydrolytic autoclaving were found in mice inoculated with different human sCJD (MM1) prions (Fig. 8). The development of mature PrP amyloid plaques in the subcallosal region (b, d, and f) was found uniquely for the vCJD inoculum.