Abbreviated incubation times for human prions in mice expressing a chimeric mouse-human prion protein transgene -- Data Supplement - Supporting Figures -- Proceedings of the National Academy of Sciences

Supporting information for Korth et al. (2003) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.2627989100

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Fig. 8.
Neuropathology of Tg22372 mice inoculated with different cases of sporadic Creutzfeldt–Jakob disease (sCJD) (MM1). Columns correspond to different inocula: (a, e, i) sCJD(MM1) (EC); (b, f, and j) sCJD(MV1) (WP); (c, g, and k) sCJD(MM1) (RG); (d, h, and l) second passage of sCJD(MM1) (RG). Rows correspond to different staining techniques: hematoxylin and eosin (Top); α-GFAP immunostaining (Middle); or α-PrP immunostaining (Bottom). The caudate nucleus is depicted, at 25´ objective. The neuropathological lesion profile after first passage of sCJD shows minimal differences in the degree of vacuolation (compare a, b, and c), intensity of astrocytic gliosis (compare e, f, and g), and PrP immunostaining after hydrolytic autoclaving (compare i, j, and k). Furthermore, there is no change in the neuropathological lesion profile after second passage (compare d, h, and l with the others).