Supporting information for Calvillo et al. (2003) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.0630444100
Fig. 5.
(A–C) Effects of hypoxia in the absence of Hepes on primary adult rat cardiomyocyte apoptosis. (D–F) rhEpo significantly improved cell survival. (A and D) Illustration of nuclei by propidium iodide (PI) labeling (blue fluorescence). (B and E) Apoptotic nuclei detected by hairpin 1 (green fluorescence). (C and F) Myocytes stained by α-sarcomeric actin (red fluorescence). Bright nuclei in myocytes reflect the combination of PI and hairpin 1 labeling of apoptotic cells, and arrowheads indicate some cells that are undergoing apoptosis. Blue nuclei in myocytes correspond to viable undamaged cells. (Confocal microscopy; scale bar = 20 µm.)