Supplementary material for Klucken et al. (2000) Proc. Natl. Acad. Sci. USA 97 (2), pp. 817-822.
Table 3.
Primer sequences and accession numbers for ABC transporters that were tested for lipid import- and export-dependent regulation in human macrophagesABC-transporter | Forward primer, | Reverse primer, | GenBank/ |
ABCA1 (ABC1) | GTTGGAAAGATTCTCTATACACCTGA | CGTCAGCACTCTGATGATGGCCTG | AJ012376 |
ABCA2 (ABC2) | GGTTCTACTCACGGCTCAAGAGCAT | CATGCTTGCGGATGAACTGGGACA | U18235 |
ABCA3 (ABC3) | GCCCTCTTTACACTCAGTTTTCA | GACGAGCAGTTGTCGTACCTAAT | U78735 |
ABCA4 (ABCR) | TGGTCAAAGCCTGGGAAGAAGTA | TCCAGGGATACATGTCAGGGAAT | AF000148 |
ABCA5 (EST90625) | TTGGTGGAACGAACACAAGAAGA | AGCAAAACACACAACCACAGCAT | U66672 |
ABCA6 (EST155051) | GGATTGCTAGGACCCAATGGTGCT | ACTCTTCTAATTTGTGAAAGGTC | U66680 |
ABCA7 | AGGTGAGCCCGGATCTTGAAACAG | CCTTGGTCAAGTTCCTCAGCA | Unpublished |
ABCB1 (MDR1) | CATTGGTGTGGTGAGTCAGG | TCGCTTTCTGTGTCCAAGG | M14758 |
ABCB2 (TAP1) | GGCTCCAAGAGCGAAAACGCAGGT | GACCATGGTGAGGGACACTGATC | HS692189 |
ABCB3 (TAP2) | GGCTGCCAGGACTATGGCTGGAGG | AGGCTTCGCAAGAGCACGTTGGCAT | HS692189 |
ABCB4 (MDR3) | GAGGATGGAGTAATTGTGGAGC | ATATGACTGAAAATGCCGGC | M23234 |
ABCB5 (EST422562) | AAACCTCACCCTGACCTCCT | CTGGTCCATAAAGCCAGCAT | U66692 |
ABCB6 (EST45597) | AGAACGTGCACTTCAGCTATGCCG | AGTTGAGAGCCTGTGTGCCACTAC | AF070598 |
ABCB7 (ABC7) | TGCGGTGCCCAGTTTGCTTTGGTAAC | CTTTTCCCTGACCCACTACCTCCTAC | AB005289 |
ABCB8 (EST328128) | TTCAACTGCATGGTCTTTGGGTA | GCTGAAGCAGACGTTCTGAAATG | U66688 |
ABCB9 (EST122234) | CCAATCTAAGCCAGTCTCACTGT | AGAGAATGCATCTCATGAAGGTC | U66676 |
ABCB10 (EST20237) | CATTCCGTCAGGATCTGTCAC | CTTGGTCAAGAACAGCAACCAT | U18237 |
ABCB11 (BSEP, sPGP) | ATTCCAGGAAAAGCATGTGTGAA | AATCCCAACAGGAAACCACAGAT | AF091582 |
ABCC1 (MRP1) | AGTGGAACCCCTCTCTGTTTAAG | CCTGATACGTCTTGGTCTTCATC | L05628 |
ABCC2 (MRP2) | ACCTGAGCTGGATCTGGTCCTCA | TATACACACTTTTGTATGTATTCT | U49248 |
ABCC3 (MRP3) | ATCAGACAGAGATTGGAGAGAAGG | CTCAGCTGTCTCATAAACTGCTTC | Y17151 |
ABCC4 (MRP4) | CCATTGAAGATCTTCCTGG | GGTGTTCAATCTGTGTGC | AF071203 |
ABCC5 (MRP5) | GAGAATGGGGATAACTTCTCAGTG | TCTCCCTAAAAGTGAAACACACAA | AF104942 |
ABCC6 (MRP6) | CGAGAGGAAAAAGGGAGTCCAGT | TCCTCATCCACTCCTTTTCAAGC | U91318 |
ABCC7 (CFTR) | TAATGCCCTTCGGCGATGTT | GCGATCCACACGAAATGTGC | M28668 |
ABCC8 (SUR) | TGGAATCCCCACACTGTCCA | CGTTCCCCAATCTGGGTCTG | L78207 |
ABCC10 (EST182763) | TTCTTCAATGCCACACCCACGGG | TGGCCGGAGCCTGTGCGGCCCAC | U66684 |
ABCD1 (ALDP) | AGGTGGAGAGGAAGAGACGCCCCCT | GGTAGGCGTGGGCCACCAGACGGCT | Z21876 |
ABCD2 (ALDR) | GGAAAATGACACATATGCTAAATG | ATACAAGTGAGCCACAGATTGG | AJ000327 |
ABCD3 (PMP70) | GAAGCCTTTACCACTGCT | CCTGCTGTGTAATTATGGG | X58528 |
ABCD4 (P70R, PMP69) | TGTGCCTGACCCTACTGGAGCAATTT | TTGCTGACCAGGGTGCATAGCTCT | Y14318 |
ABCE1 | GGTTTGCCAAGTATGCCAGT | TGTTGCCTTTAGGGAAGTGG | X74987 |
ABCF1 (TSAP, ABC50) | AGGAACAGCAGCCTGCACTC | TTGCCATTGGGTCCTACCAG | AF027302 |
ABCF2 (EST133090) | CCAATATCATTCACATGCACAAC | ACATGAGAGTGTTTTCGGATCAT | AJ005016 |
ABCF3 (EST201864) | TGCCCCACTTCTACATTCTGGAT | CTGGTCAAATCCTCCTTCCACAC | U66685 |
ABCG1 (ABC8) | CAGTGACAGCCATCCGGTGCT | CGATGAAGTCCAGGTACAGCTTGGC | U34919 |
ABCG2 (EST157481) | GAGATCATAGAGCCTTCCAAGCA | CAATTGCTGCTGTGCAACAGTGT | AF103796 |
Huwhite2 | ACATCGAGTTCGTGGAGCTGT | TTCAGGTGTCCATACTCGCCA | Unpublished |
EST1133530 | GGGAATTGTTAGCAATGCCCT | TCACTGATGCTGCTTATGCC | Unpublished |
EST168043 | CTCTAAACTGGGTGTTCTCCT | TCGCAGCACGGGAAACAGATT | Unpublished |
EST640918 | CCAGGCTGCTCAGCAGGAAAG | GTACCACCTCTGATATAAGGC | Unpublished |
EST990006 | GGAAGATGTGCAACCTTTAGC | CGGGTTTAAACAGGAACACAG | Unpublished |
EST698739 | CACAGGCTGCTTGGCAGGAAA | GGGGATGAATAACATGTCTGA | Unpublished |
GAPDH | CCCTTCATTGACCTCAACTACAT | ACGATACCAAAGTTGTCATGGAT | J02642 |
The new designations for ABC transporters according to the suggestions of the Human Gene Nomenclature Committee (http://www.gene.ucl.ac.uk/users/hester/abc.html) were used.