Supporting information for Morin et al. (2003) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.0831036100

 

Table 2. Primers used to analyze the pink bollworm cadherin gene (BtR)

Primer name

Primer sequence

cDNA Position, bp

Cad(-106)

5'-AGT GCG AGT GCT TTG AAT CTG-3'

(–106)-(–86)

Cad1381

5

'-GGC TTC CAG AAC CAG ACA TTC-3'

1381–1401

Cad1441

5

'-CCT CAT AAT CCA GCA TTC GGT-3'

1441–1421

Cad3221

5

'-TGT CGC CTA ACA ACG TAA CCG-3'

3221–3241

Cad3324

5

'-TCA GCA GAT ATC TCA TGA GGT GT-3'

3324–3346

Cad3386

5

'-AGT TCG TTT TTC CTG AAT CCG G-3'

3386–3407

Cad3645

5

'-AAA TTC CTT GCC TTC CTC AGG-3'

3645–3625

Int540

5

'-CTA ACT ATT TGC TTC TGA CTG-3'

Marker intron

Oligo(dT)

(dT)20

poly(T) tail

Three overlapping fragments of the BtR gene were reversed-transcribed and amplified using the primer pairs Cad(-106) and Cad1381; Cad1441 and Cad3645; and Cad3221 and oligo(dT). Forward primers Cad3324 or Cad3386 and backward primer Int540 amplified the first 540 bp of the "marker" intron.