Liu et al. 10.1073/pnas.0602923103. |
Supporting Table 1
Supporting Figure 8
Supporting Figure 9
Fig. 8. p42 promotes PC12 cell differentiation, but p48 suppresses it. Control (Top), p42 (Middle), and p48 (Bottom) PC12 cells (1 ´ 105) were maintained in tetracycline-free medium. After 24 h, NGF (50 ng/ml) was introduced into the medium. At the indicated time, the cell morphology was examined under a microscope.
Fig. 9. The expression of GST-Ebp1 and ErbB3 was verified by immunoblotting.
Table 1. Cell-cycle analysis of Ebp1 stable cell lines with FACS analysis
Cell lines | Treated with NGF, days | % G1 | % S | % G2/M |
Control | 0 | 50 (5.7) | 20 (3.1) | 20.5 (2.1) |
3 | 43 (3.8) | 21.5 (3.5) | 22 (1.8) | |
5 | 45.7 (4.1) | 21 (2.8) | 23.5 (2.3) | |
P48-Ebp1 | 0 | 54.4 (5.5) | 31.2 (3.9) | 9.89 (0.5) |
3 | 57.3 (6.2) | 27.7 (3.1) | 10.5 (0.7) | |
5 | 58.5 (6.1) | 27.1 (2.6) | 10.1 (0.5) | |
P42-Ebp1 | 0 | 64.1 (5.2) | 19.5 (2.1) | 13.9 (1.6) |
3 | 69.2 (5.4) | 15.4 (2.6) | 11.9 (1.2) | |
5 | 69.5 (5.4) | 15.9 (1.9) | 12.1 (1.5) |
The data in parentheses are SD from triplicate experiments.