Bowers et al. 10.1073/pnas.0605789103. |
Table 2. Quantitative PCR mRNA expression profiling of proliferating 10T1/2 stem cells and A-33 preadipocytes reveals differential expression of several BMP 2/4/7 family signaling pathway components
Class | Gene | Aliases | Fold increase in A33 |
Ligands | BMP-4 | Bone morphogenetic protein-4 | 7.1 |
Receptors | BMPr1a | BMP receptor type 1a; ALK3 | 2.1 |
BMPr1b | BMP receptor type 1b; ALK6 | Only in 10T1/2 cells | |
BMPr2 | BMP receptor, type II | 2.1 | |
Intracellular signal transducers | Smad1 | MAD homolog 1 | 2.5 |
Smad4 | MAD homolog 4 | 2.7 | |
Smad5 | MAD homolog 5 | 1.8 | |
Smad9 | MAD homolog 9; Smad8 | 9.4 | |
BMP target genes | Id1 | Inhibitor of DNA binding 1 | 4.3 |
Id2 | Inhibitor of DNA binding 2; Idb2 | 3.3 | |
Id3 | Inhibitor of DNA binding 3, Idb3 | 6.3 | |
Msx2 | Homeobox, msh-like 2 | -10.3 | |
Extracellular modulators | BMP-1 | Bone morphogenetic protein-1 | 1.8 |
Chrd | Chordin | 8.1 | |
Chrdl1 | Chordin-like 1; neurogensin-1 | 55.7 | |
Grem1 | Gremlin-1 | -8.2 | |
Htra1 | Htra serine peptidase 1; Prss11 | 28.5 | |
Nog | Noggin | -3.6 | |
Twsg1 | Twisted gastrulation homolog 1 | 2.0 |
mRNA levels were normalized to GAPDH levels by the DDCt method and are expressed as fold increase in A33 cells (+ values means A33 > 10T1/2). All differences are statistically significant (P > 0.05)