Genomewide transcriptional changes associated with genetic alterations and nutritional supplementation affecting tryptophan metabolism in Bacillus subtilis -- Data Supplement - Supporting Information -- Proceedings of the National Academy of Sciences

Supporting information for Berka et al. (2003) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.1031606100

Supporting Text
RNA Isolation.
RNA was prepared by using the Bio101 FastRNA Blue Kit (Qbiogene), according to the manufacturer’s instructions, with minor modifications. Two hundred microliters of cell suspension was added to each FastPrep blue tube containing the lysing matrix and the other required reagents. These mixtures were shaken in the FastPrep instrument at a speed rating of 6, two times for 45 s and then for an additional 30 s. The rest of the procedure was as described (Qbiogene). Typically, 200–300 μg of RNA was obtained from each culture. Each RNA sample was analyzed on agarose gels to assess quality. In all cases, 23s rRNA signals were detected with greater intensity than the 16s rRNA bands.