Mutation or Drug-Dependent Microtubule Disruption Causes Radial Swelling without Altering Parallel Cellulose Microfibril Deposition in Arabidopsis Root Cells -- Sugimoto et al. 15 (6): 1414 Data Supplement

Mutation or Drug-Dependent Microtubule Disruption Causes Radial Swelling without Altering Parallel Cellulose Microfibril Deposition in Arabidopsis Root Cells
Plant Cell Sugimoto et al. 15 (6): 1414.

Supplemental Data

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Supplemental Figure 1 - Treatment of wild-type Arabidopsis roots with taxol for 24 h results in the loss of overall, but not local, alignment of cellulose microfibrils. (A) Field emission scanning electron micrograph of a 5-day-old mor1-1 root tip after 24-h treatment with 10 µM taxol. The radially swollen root tip has been cryo-planed to expose the inner cell walls for examination. Bar = 100 μm. (B) to (D) Cellulose microfibril texture in three sites from a radially swollen cell in the root elongation zone. Though locally parallel, microfibrils have variable orientation. Bar = 300 nm.
Supplemental Figure 2 - Cortical microtubule arrays in the mor1-1rsw1-1 double mutant become disordered to the same extent as those in the mor1-1 single mutant, whereas rsw1-1 and wild-type microtubule arrays remain intact. Seedlings were shifted to 29°C for 4 h prior to processing the root tips for anti-tubulin immunofluorescence. Bar = 20 μm. (A) wild-type (Col-0). (B) mor1-1. (C) rsw1-1. (D) mor1-1rsw1-1.