Inaugural Article: Patterns of nucleotide misincorporations during enzymatic amplification and direct large-scale sequencing of ancient DNA -- Stiller et al. 103 (37): 13578 Data Supplement - HTML Page - index.htslp -- Proceedings of the National Academy of Sciences

Stiller et al. 10.1073/pnas.0605327103.

Supporting Information

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Supporting Figure 5
Supporting Figure 6
Supporting Figure 7
Supporting Table 1

Fig. 5. Geographical distribution of the 29 wolf samples from which canid-like mitochondrial DNA could be amplified repeatedly. Numbers 1-14 refer to the sampling locations. 1, Goyet Cave, Belgium; 2, Trou des Nutons Cave, Belgium; 3, Frankenthal near Rhein River, Germany; 4, Eich near Rhein River, Germany; 5, Ginsheim near Rhein River, Germany; 6, Vypustek near Brno, Czech Republic; 7, Slouper Cave near Brno, Czech Republic; 8, Istallosko Cave near Budapest, Hungary; 9, Nerubajskoe near Odessa, Ukraine; 10, Zaskalnaya on Crimean Peninsula, Ukraine; 11, Zamyatino near Lipetsk, Russia; 12, ZIN 32173, Turkmenistan; 13 Razboinichya Cave in Altai Mountains, Russia; 14, Tronny Grotto on Sakhalin Island, Russia.

Supporting Figure 6

Fig. 6. Six oligonucleotides used as templates to determine misincorporation rates of different DNA polymerase reagents. The oligonucleotide pairs carry an A and its deaminated form hypoxanthine (H); a C and its deaminated form uracil (U); and a G and its deaminated form xanthine (X), respectively.

Supporting Figure 7

Fig. 7. Comparison of misincorporation patterns generated by two different polymerase reagents (AmpliTaq Gold and Platinum Taq High Fidelity). Shown are the numbers of misincorporations seen in clone sequences of amplifications from three ancient DNA extracts (wolf samples 21, 22, and 26 of Table 1), which were amplified both with AmpliTaq Gold and Platinum Taq High Fidelity in parallel. The number of misincorporations is derived from 333 clones in case of the AmpliTaq Gold and 321 clones for the Platinum Taq High Fidelity. The misincorporation patterns of the two polymerase reagents differ from each other (χ² = 11.6; df = 4; P = 0.02).