Supporting Figures

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Supporting Figure 4
Supporting Figure 5

Fig. 4. Sequence alignment of the miR-146 family of miRNAs. All sequences are taken from microRNA registry (release 7.1) (1). Variable nucleotides are shown in red.

1. Griffiths-Jones, S. (2004) Nucleic Acids Res. 32, D109-D111.

Fig. 5. 3' UTR sequences, as determined by Ensembl (www.ensembl.org), of miR-146a/b target genes TRAF6, IRAK1, and COT/Tpl2/MAP3K8. Portions of 3' UTRs analyzed in reporter assays are shown in uppercase lettering. miR-146 hexamer seed matches were predicted by TargetScan (1), TargetScanS (2) (bold), Miranda (3) (italicized), or Pictar (4) (underlined). In the mutant versions of each reporter, miR-146 seeds conserved across five mammalian species (highlighted in red) were changed to GAAGAC (Fig. 3). All reporter plasmids were constructed by using pMIR-REPORT (Ambion).

1. Lewis, B. P., Shih, I. H., Jones-Rhoades, M. W., Bartel, D. P. & Burge, C. B. (2003) Cell 115, 787-798.

2. Lewis, B. P., Burge, C. B. & Bartel, D. P. (2005) Cell 120, 15-20.

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