Characterization of capsaicin synthase and identification of its gene (csy1) for pungency factor capsaicin in pepper (Capsicum sp.) -- Prasad et al. 103 (36): 13315 Data Supplement - HTML Page - index.htslp -- Proceedings of the National Academy of Sciences

Prasad et al. 10.1073/pnas.0605805103.

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Supporting Figure 7
Supporting Figure 8
Supporting Figure 9

Supporting Figure 7

Fig. 7. PCR amplification of capsaicin synthase gene (csy1) using degenerate primer designed based on N-terminal amino acid sequence as forward primer and placental tissue-specific acyl transferase as reverse primer. Lane M, Marker; lanes A-C, PCR amplification of csy1 using cDNA as template; lanes D-F, PCR amplification of csy1 using genomic DNA as template

Supporting Figure 8

Fig. 8. Coding sequence of capsaicin synthase gene with deduced amino acid sequence. The csy1 encoded for 308-aa sequences, followed by 3' UTR region.

Supporting Figure 9

Fig. 9. Western blot with CS antibody for recombinant capsaicin synthase. Lane A, Western blot with preimmune sera (Control); lane B, Western blot with native CS from placenta of Capsicum fruits; lane C, Western Blot with recombinant CS from Escherichia coli.