GDC ******** GeneDoc Multiple Sequence Alignment Editor ******** GDC 0109f50190000cffffff00000000000000000000000000ff00c0c0c0ffff GDC ff000000000000f43f000000000000f43f000000000000f03f0000000000 GDC 00f03f00A000300000190000cA54696d6573204e657720526f6d616e00A0 GDC 0820002000000000000000000000601444e000245510003535400044b520 GDC 00546595700064c49564d002848756d616e46414b2c0d0a4368696d70464 GDC 14b2c0d0a52617446414b2c0d0a4d6f75736546414b2c0d0a436869636b6 GDC 56e46414b2c0d0a46726f6746414b2c0d0a5a65627261666973683161464 GDC 14b2c0d0a5a6562726166697368316246414b2c0d0a46756775316e65774 GDC 6414b2c0d0a46756775326e657746414b2c0d0a48756d616e50796b322c0 GDC d0a4368696d7050796b322c0d0a52617450796b322c0d0a4d6f757365507 GDC 96b322c0d0a436869636b656e50796b322c0d0a46726f6750796b322c0d0 GDC a5a656272616669736850796b32312c0d0a5a656272616669736850796b3 GDC 2322c0d0a4675677550796b322c0d0a43696f6e6146414b2c0d0a5365615 GDC 5726368696e46414b2c0d0a44726f736f7068696c6146414b2c0d0a416e6 GDC f706861656c6546414b2c0d0a4170697346414b2c0d0a43656c6567616e7 GDC 346414b2c0d0a48796472612c0d0a48796472616374696e6961293b0d0a0 GDC 0CBAABAAA0000002a000a00020001B20AAA000e203a2000203a200000000 GDC 000000059400000000000000000000c5058000000ffff000047580000ff0 GDC 0ff000041475358000000ffffff0056434147444e535450580000ff00fff GDC f00484b525800ff00000000ff4544580000ff000000ff484b5245445800f GDC fffff0000ff48524551444e425a5800ff000000ff005957484b524551444 GDC e5354425a580000000000ff00494c56415800ff000080808046595748580 GDC 00000ff808080494c564341474d4659574854505800ffffff0000000c505 GDC 8000000000a00004758000000008c8db3414347535800000000000000564 GDC 147444e53545058001300d1010108484b52580001000050ceb3454458001 GDC 87ec7b80500484b524544580000000000000048524551444e425a58002d0 GDC 000010000435957484b524551444e5354425a5800000000000000494c564 GDC 15800000000110000465957485800000000000000494c564341474d46595 GDC 74854505800d8c8500000000300ffffff00000000ffffff8080800000000 GDC 0c0c0c00000ABBBBBBBBBBBBBB00ff00A001b00010001000100010001000 GDC 100010001000100010001000100010001000100010001000100010001000 GDC 1000100010001000100010001A0018AA000000000000000000000003A00f GDC f00A00000000008056400000000000805140000000000000494000000200 GDC 00000000001440A0000000098b20c4100000000102506410000000018980 GDC 6410000000078b10b4100AAABB00005374616e646172640046464c4c5353 GDC 535359592a2a43432a574c4c4c4c5050505048485151525252524949494d GDC 545454544e4e4b4b5353525256565656414141414444454547474747002d GDC 2d2d2d2d2d2d2d2d2d2d2d2d2d2d2d2d2d2d2d2d2d2d2d2d2d2d2d2d2d2d GDC 2d2d2d2d4d2d2d2d2d2d2d2d2d2d2d2d2d2d2d2d2d2d2d2d2d2d2d2d2d2d GDC 2d2d2d00BBBBB000000000000001b0000000000000000000000000000000 GDC 000000000000000000000000000000000000000000000000000000000000 GDC 000000000000000000003000000000000BBAB001b0000000000000000000 GDC 000000000000000000000000000000000000201b7A000000000000594000 GDC 000000000054400000000000004e4000DABB000000ffffff030201444548 GDC 4b524e5153540000ff00ff0000024c49564d4659574147435000ff000000 GDC ff0004014445484b520000ff00ff0000024e5153540000ff00ff8000034c GDC 49564d46595700ff000000ff0004414700ff0000008000080144450000ff GDC 00ff000002484b5200ffffffff0000034e510000ff00ff800004535400ff GDC ffffff8000054c495600ff000000ff000646595700ffffff00ff00074147 GDC 00ff0000008000084d4300ffffff0080000000000000Affffff80808000A augustv4.msf MSF: 1556 Type: P August 3, 2006 10:00 Check: 9967 .. Name: HumanFAK Len: 1556 Check: 5802 Weight: 1.00 Name: ChimpFAK Len: 1556 Check: 5311 Weight: 1.00 Name: RatFAK Len: 1556 Check: 4888 Weight: 1.00 Name: MouseFAK Len: 1556 Check: 4922 Weight: 1.00 Name: ChickenFAK Len: 1556 Check: 2490 Weight: 1.00 Name: FrogFAK Len: 1556 Check: 9869 Weight: 1.00 Name: Zebrafish1aFAK Len: 1556 Check: 235 Weight: 1.00 Name: Zebrafish1bFAK Len: 1556 Check: 1521 Weight: 1.00 Name: Fugu1newFAK Len: 1556 Check: 9488 Weight: 1.00 Name: Fugu2newFAK Len: 1556 Check: 8094 Weight: 1.00 Name: HumanPyk2 Len: 1556 Check: 3700 Weight: 1.00 Name: ChimpPyk2 Len: 1556 Check: 3131 Weight: 1.00 Name: RatPyk2 Len: 1556 Check: 4722 Weight: 1.00 Name: MousePyk2 Len: 1556 Check: 6418 Weight: 1.00 Name: ChickenPyk2 Len: 1556 Check: 4107 Weight: 1.00 Name: FrogPyk2 Len: 1556 Check: 179 Weight: 1.00 Name: ZebrafishPyk21 Len: 1556 Check: 3172 Weight: 1.00 Name: ZebrafishPyk22 Len: 1556 Check: 3925 Weight: 1.00 Name: FuguPyk2 Len: 1556 Check: 1790 Weight: 1.00 Name: CionaFAK Len: 1556 Check: 6627 Weight: 1.00 Name: SeaUrchinFAK Len: 1556 Check: 6902 Weight: 1.00 Name: DrosophilaFAK Len: 1556 Check: 362 Weight: 1.00 Name: AnophaeleFAK Len: 1556 Check: 5639 Weight: 1.00 Name: ApisFAK Len: 1556 Check: 258 Weight: 1.00 Name: CelegansFAK Len: 1556 Check: 6296 Weight: 1.00 Name: Hydra Len: 1556 Check: 7995 Weight: 1.00 Name: Hydractinia Len: 1556 Check: 2124 Weight: 1.00 // 1 50 HumanFAK .......... ......MAAA YLDPNLNH.. TPNSSTKTH. .......... ChimpFAK .......... ......MAAA YLDPNLNH.. TPNSSTKTH. .......... RatFAK .......... ......MAAA YLDPNLNH.. TPSSSTKTH. .......... MouseFAK .......... ......MAAA YLDPNLNH.. TPSSSTKTH. .......... ChickenFAK .......... ......MAAA YLDPNLNH.. TPSSSAKTH. .......... FrogFAK .......... ......MAAA YLDPNLNH.. NPSTNAKSR. .......... Zebrafish1aFAK .......... ......MAAA YLDPNPTH.. ALNQGVKPR. .......... Zebrafish1bFAK .......... ......MATA FLEPTPNH.. TLSVGAKAR. .......... Fugu1newFAK PEYDRHLAAA VKAGKTMATA YLDPNLNH.. AFLGGAKSR. .......... Fugu2newFAK .......... .......SSS YLEPNHNHSA ASGCGVKSRS GMPSNTGVGT HumanPyk2 .......... .......MSG VSEPLSRVKL GTLRRPEGPA EPMVVVPVD. ChimpPyk2 .......... .......MSG VSEPLSRVKL GTLRRPEGPA EPMVVVPVD. RatPyk2 .......... .......MSG VSEPLSRVKV GTLRPPEGPP EPMVVVPVD. MousePyk2 .......... .......MSG VSEPLSRVKV GTLRRPEGPP EPMVVVPVD. ChickenPyk2 .......... .......... .......... .......... .......... FrogPyk2 .......... .......... .......... .......... .......... ZebrafishPyk21 .......... .......... MASDASTLSW KVPAQDWDGG IKSPDLGPVG ZebrafishPyk22 .......... .......... .......... .......... .......... FuguPyk2 .......... ........MY EVMSGDTLSW KVPSPRQSEA TPESHFTVD. CionaFAK .......... .......... .......... .......... .......... SeaUrchinFAK .......... .......... .......... .......... .......... DrosophilaFAK .......... .......... .......... .......... .......... AnophaeleFAK .......... .......... .......... .......... .......... ApisFAK .......... .......... .......... .......... .......... CelegansFAK .......... .......... .......... .......... .......... Hydra .......... .......... .......... .......... .......... Hydractinia .......... .......... .......... .......... .......... 51 100 HumanFAK .LGTG..MER SPGAMERVLK VFHYFESNSE PTTWASIIRH GDATDVRG.. ChimpFAK .LGTG..MER SPGAMERVLK VFHYFESNSE PTTWASIIRH GDATDVRG.. RatFAK .LGTG..TER SPGAMERVLK VFHYFESSNE PTTWASIIRH GDATDVRG.. MouseFAK .LGTG..MER SPGAMERVLK VFHHFESSSE PTTWASIIRH GDATDVRG.. ChickenFAK .LGTG..MER SPGAMERVLK VFHYFENSSE PTTWASIIRH GDATDVRG.. FrogFAK .LSTG..MER SPGAIERVLR VFHYFESNNE PATWSSNIRH GDATDVRG.. Zebrafish1aFAK .FSAG..MER TAAALERVLK IFHYFESSSE PCSWASNIRH GDSTDVRG.. Zebrafish1bFAK .LSAGT..ER VPGAPDRVLK VFHHFENNSE HSTWSSNIRH GDATDVRG.. Fugu1newFAK .LSAGGSDRT VAGSVDRVLK VFHHFETNTE QNFWSSNIRY GDATDVRG.. Fugu2newFAK GGSAGGL.ER PPGSMDRVLK IFHYFETNNE PCNWASNIRH GDGTDVRG.. HumanPyk2 .......VEK ...EDVRILK VC..FYSNSF NPG....... KNFKLVKCTV ChimpPyk2 .......VEK ...EDVRILK VC..FYSNSF NPG....... KNFKLVKCTV RatPyk2 .......VEK ...EDVRILK VC..FYSNSF NPG....... KNFKLVKCTV MousePyk2 .......VEK ...EDVRILK VC..FYSNSF NPG....... KNFKLVKCTV ChickenPyk2 .......... .......... .......... .......... .......... FrogPyk2 .......... .......... .......... .......... .......... ZebrafishPyk21 D......... ..GGPVKIIK VC..FSSSNN .LS....... KNFKLVKCDS ZebrafishPyk22 .......... .....VKIIK VC..FISNSA NLG....... KNFKLVKCES FuguPyk2 .......... ..SEAVKILK VC..FSTNNS .LG....... KNFKLVKCDS CionaFAK .......... .......... .......... .......... .......... SeaUrchinFAK .......... .......... ...MASKARE KMMLKVYQPN GSFNSVKCME DrosophilaFAK .......... MNTAGATSQP PPTKNEINSE EYLIHVHMPN KSFKAVRFNV AnophaeleFAK .......... .......... .......... ...LHVYLPN HGFRMIRFDE ApisFAK .......... .......... ........MD KATLKVHLPN GGFNVVKFGD CelegansFAK .......... .......... .........M EGLARVFLIG GASKAVRYDE Hydra .....MPVSN PSRNDTGINP TKLERHQSTL NSMLHVFLPN GEFRAVRFGD Hydractinia ....MPEVVT QKRNSAGISP TKLERHQSAL KTTLHVFLSN GEFRAVKFGD 101 150 HumanFAK ......IIQK IVDSHKVKHV A.....CYGF RLS......H LRSEEVHWLH ChimpFAK ......IIQK IVDSHKVKHV A.....CYGF RLS......H LRSEEVHWLH RatFAK ......IIQK IVDSHKVKHV A.....CYGF RLS......H LRSEEVHWLH MouseFAK ......IIQK IVDSHKVKHV A.....CYGF RLS......H LRSEEVHWLH ChickenFAK ......IIQK IVDCHKVKNV A.....CYGL RLS......H LQSEEVHWLH FrogFAK ......IIQK IVDSHKVKNV A.....SYGL RLS......H LHSEEVHWLH Zebrafish1aFAK ......IIQK ILDIHKVRCV S.....CFGL RLS......H LRTGEIHWLH Zebrafish1bFAK ......IIQK IVDIHKVQHV V.....CFGL RLT......H VPSGDVHWLH Fugu1newFAK ......IIQK ILDIHKVRFT S.....CFGL RLSN.....S QFRDQVHWLH Fugu2newFAK ......VIQK IAEIHKLRCV S.....SLGL RLT......H LHSDALHWLH HumanPyk2 QTEIREIITS ILLSGRIGPN IRLA.ECYGL RLK......H MKSDEIHWLH ChimpPyk2 QTEIREIITS ILLSGRIGPN IQLA.ECYGL RLK......H MKSDEIHWLH RatPyk2 QTEIQEIITS ILLSGRIGPN IQLA.ECYGL RLK......H MKSDEIHWLH MousePyk2 QTEIQEIITS ILLSGRIGPN IQLA.ECYGL RLK......H MKSDEIHWLH ChickenPyk2 .......... .......... .......... .......... .......... FrogPyk2 .......... .......... .......... .......... .......... ZebrafishPyk21 SWQIKTIIQS ILISGRLGPN VQNP.GCFGL RLK......H LKSEELHWLH ZebrafishPyk22 SWNIRSIIKA ILDSGRLGPN IKCF.ECYGL MLK......H LKSDEVHWLH FuguPyk2 SWQVRAIIRS ILNCGRLGPD IKHV.GCYGL LLK......H LKSDELHWLH CionaFAK .......... .........V D.....CFGL RLR......H MKTGDVFWLH SeaUrchinFAK TTDIKNVIHA VVGKLARGER AFES.SFAVR LQH......T LS.EECHWLH DrosophilaFAK KETVFHVIRR TVEDLGTDGR TPSI.QRYAC RML......N MITKEVIWLA AnophaeleFAK ASDVRQIIN. LIVGWMSPGQ KPNP.QSYAL RLR......H MLTKEVLWMP ApisFAK AIDVKGIIT. LVTSRLAVGT RHYH.NLYAM RLH......H PGSGESYWLH CelegansFAK QTTIERVI.H VVA.RGIGIS QVAV.AHFAL RLVTGPSPQT AGSGDSLWLH Hydra NSDMKSIVET .VIR.RLGAN ISVASKFYAL KLE......N ILNKESFWLS Hydractinia NSDLKSIIDI .VTR.RLGAN ISLSSKFYGI KFE......H VETKAFFWLN 151 200 HumanFAK VDMGVSSVR. EKYELA.... .......... .......... ....HPPEEW ChimpFAK VDMGVSSVR. EKYELA.... .......... .......... ....HPPEEW RatFAK VDMGVSSVR. EKYELA.... .......... .......... ....HPPEEW MouseFAK VDMGVSSVR. EKYELA.... .......... .......... ....HPPEEW ChickenFAK LDMGVSNVR. EKFELA.... .......... .......... ....HPPEEW FrogFAK PDIGVSHIR. EKYEQS.... .......... .......... ....HPPEEW Zebrafish1aFAK PDMGVSHVR. ERYEHS.... .......... .......... ....HPQDDW Zebrafish1bFAK PDEGVSHVR. EKFEQK.... .......... .......... ....RPQDEW Fugu1newFAK PEMGVSHVR. EKYEQA.... .......... .......... ....RPTEEW Fugu2newFAK PDLGVSHIR. EKYEKQ.... .......... .......... ....HPQDEW HumanPyk2 PQMTVGEVQ. DKYECL.... .......... .......... ....HVEAEW ChimpPyk2 PQMTVGEVQ. DKYECL.... .......... .......... ....HVEAEW RatPyk2 PQMTVGEVQ. DKYECL.... .......... .......... ....HVEAEW MousePyk2 PQMTVGEVQ. DKYECL.... .......... .......... ....HVEAEW ChickenPyk2 .......... .......... .......... .......... .......... FrogPyk2 .......... .......... .......... .......... .......... ZebrafishPyk21 PDLTVGEVE. QRYESH.... .......... .......... ....HAEAEW ZebrafishPyk22 PKLTVAEVE. QKYEQQ.... .......... .......... ....HVEAEW FuguPyk2 PDLTVEEVE. QRYESH.... .......... .......... ....HVEAEW CionaFAK FNQTMGEVKE KYEII..... .......... .......... ....HPIDEC SeaUrchinFAK RDLTVGQVK. KKYEAY.... .......... .......... ....HPAIEW DrosophilaFAK RSTSMQKVLS HILTPGCSNV DCPNNQSELD EVLLEHGRR. ...ITDNRVW AnophaeleFAK PDTSMSQVMA HIYNPSCSNA DCPNV.DKST IAKRMQQKTS GAVGHANSVW ApisFAK QDTTMYQVQE KYEKK..... .......... .......... ....HPHCEW CelegansFAK PMLRITQL.P HIYARHLPI. .......... .......... ....GVCDEI Hydra SSLTMNEVKL KYEKEHNNEN .......... .......... .........W Hydractinia PALTMTDVKN KFENEHKDEK .......... .......... .........W 201 250 HumanFAK KYELRIRYLP KGFLNQFTED KPTLNFFYQQ VKSDYMLEIA DQ......VD ChimpFAK KYELRIRYLP KGFLNQFTED KPTLNFFYQQ VKSDYMLEIA DQ......VD RatFAK KYELRIRYLP KGFLNQFTED KPTLNFFYQQ VKSDYMLEIA DQ......VD MouseFAK KYELRIRYLP KGFLNQFTED KPTLNFFYQQ VKSDYMQEIA DQ......VD ChickenFAK KYELRIRYLP KGFLNQFTED KPTLNFFYQQ VKNDYMLEIA DQ......VD FrogFAK KYELRIRYLP KGFVNQFTED KPTLNFFYQQ VKNDYMSEIA DQ......VD Zebrafish1aFAK RYELRIRYLP KGFLNQFTED QPTLNYFYHQ VKSDYMMEIA DQ......VD Zebrafish1bFAK RYELRIRYLP KGFLTQFTED KPTLNYFYQQ VKNDYMLEVA DQ......VE Fugu1newFAK RYELRIRYLP KSFVQQFSED KPTLSYFYHQ VKNDYMTEIG DQ......VD Fugu2newFAK RYELRIRYLP KGYLSHFSED KPSLNYLYHQ VKSDYMQHIA DQ......VD HumanPyk2 RYDLQIRYLP EDFMESLKED RTTLLYFYQQ LRNDYMQRYA SK......VS ChimpPyk2 RYDLQIRYLP EDFMESLKED RTTLLYFYQQ LRNDYMQRYA SK......VS RatPyk2 RYDLQIRYLP EDFMESLKED RTTLLYFYQQ LRNDYMQRYA SK......VS MousePyk2 RYDLQIRYLP EDFMESLKED RTTLLYFYQQ LRNDYMQRYA SK......VS ChickenPyk2 .......... .......... .......... .....MQNYA SK......VS FrogPyk2 .......... .......... .......... .....MQQYA TK......VS ZebrafishPyk21 RYDLRIRYIP IDYMKRLRED RTFLLYFYQQ VRSDYMQHHA SK......VS ZebrafishPyk22 RYDLRIRYIP SDFLEKLKDD KTTMLYFYQQ VRADYMQHNA RR......VS FuguPyk2 RYDLRIRYVP VNFLEKFSDD RSTLLYFYQQ VRSDYMQSHA CK......VS CionaFAK KYELRVRYIP RNLQEMLEKD RNQGQVPRIY YGMFFQVYSD YLEAIAEKVD SeaUrchinFAK KYELRVRYLP KNYHELLEKD KVSFFYFYDQ VRCDYMNEVA EL......VD DrosophilaFAK RVELRVRYVP NNIQELFEED KATC.FYYFN QVKEDFIQAN VT.....AID AnophaeleFAK KAELRVRYIP KNLKELYERD RTTCHFYFDQ VKQDYIQSNV PH......ID ApisFAK RYELRVRYLP QNLNDLYEKD KVTFYYYYDQ VRNDYLCANH AA......LD CelegansFAK KLEMRMRFMP QSVYELQATD SSAFVYLHEQ VVDEFFSHVA WR......SS Hydra RFNLRVRFIT SSFTDLYNTD KTTFYFLYDQ VRSDYMKNLA EQ......VD Hydractinia RYNLRIRYLP IVFKDLYTID KTTFYFLYDQ IRTDYMKNIA ET......VE 251 300 HumanFAK QEIALKLGCL EIRRSYWEMR GNALEKKSNY EVLEKDVGLK RFFPKSLLDS ChimpFAK QEIALKLGCL EIRRSYWEMR GNALEKKSNY EVLEKDVGLK RFFPKSLLDS RatFAK QDIALKLGCL EIRRSYWEMR GNALEKKSNY EVLEKDVGLK RFFPKSLLDS MouseFAK QEIALKLGCL EIRRSYWEMR GNALEKKSNY EVLEKDVGLK RFFPKSLLDS ChickenFAK QEIALKLGCL EIRRSYGEMR GNALEKKSNY EVLEKDVGLR RFFPKSLLDS FrogFAK QEIALKLGCL EIRRSYGEMR GNALEKKSNY EVLEKDVGLK RFFPKSLLDS Zebrafish1aFAK QDIALKLGCL EIRRFFRDMP GNALDKKSNY ELLEKDVGLR RFFPKSLLDS Zebrafish1bFAK QEVALKLGCL EIRRFYREMR GNALDKKSNY ELLEKDVGLR RFFPKSLLES Fugu1newFAK QDVALKLGCL EIRRFFKEMR GNALDKKSNY ELLEKDVGLR RFFPKALLDS Fugu2newFAK QDVALKLGCL EIRRFFREMP GNALDKKSNY ELLEKDVGLR RFFPRSLLES HumanPyk2 EGMALQLGCL ELRRFFKDMP HNALDKKSNF ELLEKEVGLD LFFPKQMQEN ChimpPyk2 EGMALQLGCL ELRRFFKDMP HNALDKKSNF ELLEKEVGLD LFFPKQMQEN RatPyk2 EGMALQLGCL ELRRFFKDMP HNALDKKSNF ELLAKEVGLD LFFPKQMQEN MousePyk2 EGMALQLGCL ELRRFFKDMP HNALDKKSNF ELLEKEVGLD LFFPKQMQEN ChickenPyk2 EGMALQLGCL ELRRFYKDMP QNALDKKSNF EFLEKEVGLD LFFPSQMQEN FrogPyk2 EGMALQLGCL ELRRFYKDMP QNALEKKSNF EFLEKEVGLD LFFPKQMQES ZebrafishPyk21 DGMALQLGCL EIRRFYKDMN AKGLEKKSNF DLLEKDVGLD LFFPKGLIDS ZebrafishPyk22 DGMALQLGCL EIRRFYKDMN ASGLEKKSNF ELLEKDVGLD LFFPPELIDS FuguPyk2 DGMALQLGCL EIRRFYKDMN AKGLEKKSNF ELLEKEVGLD LFFPQQLINS CionaFAK HDTAIKLGCL EIRRYFKDMP HSVFEKKSNF ELLERDVGLR KFLPKSVIES SeaUrchinFAK QDISLQLGCL EMRRFFKDMP QIALDKKANF DYLEKEIGLK RFIPKNVIDN DrosophilaFAK TEVAVQLCCL GIRHYFKNIT VKAPDKKQHI DYIEKEIGFK SFLPQSVIAT AnophaeleFAK PEIAVQLCCL GIRHYYKDTN HTSNDRKQHL DYIEKEMGFG NFIPKSVIDT ApisFAK QDVAVQLCCL EIRYFFKDMQ QIALDKKSNL EYLEREVGLH KFLPRSVLNG CelegansFAK VEVALEVAAL KVCRDFAEHQ HNKGADHHLE DLDIEACIQS LIPNVLHNPG Hydra PDLAFQLGVL EMKKFFNNMA QSALDKKSNL DFIEKEFGLT RFLPQNIIET Hydractinia TSLAFQLGVL EMKRFFNNMP QSALDKKSNL DFIEKEIGLE RFLPNTVLDH 301 350 HumanFAK VKA.KTLRKL IQQTFRQFAN LNREESILKF FEILSPVYRF DKECFKCALG ChimpFAK VKA.KTLRKL IQQTFRQFAN LNREESILKF FEILSPVYRF DKECFKCALG RatFAK VKA.KTLRKL IQQTFRQFAN LNREESILKF FEILSPVYRF DKECFKCALG MouseFAK VKA.KTLRKL IQQTFRQFAN LNREESILKF FEILSPVYRF DKECFKCALG ChickenFAK VKA.KTLRKL IQQTFRQFAN LNREESILKF FEILSPVYRF DKECFKCALG FrogFAK VKA.KTLRKL IQQTFRQFAN LNREESILKF FEILSPVYRY DKECFKCALG Zebrafish1aFAK VKA.KSLRKL IQQTFKQFAN LNNEQCMLKF FEILSPIYRF DKECFKCALG Zebrafish1bFAK VKA.KTLRKQ IQQTFKQFAN LNDEQSVLKF FEILAPVYRF DKECFRCALG Fugu1newFAK VKA.KTLRKL IQQTFKQVAN LNDEQCILKF LDILAPICRY DKECFKCALG Fugu2newFAK LKA.KTLRKQ IQQTFKQFAN LNDEQSIHKF FEIVSPIYRF DKECFKCALG HumanPyk2 LKP.KQFRKM IQQTFQQYAS LREEECVMKF FNTLAGFANI DQETYRCELI ChimpPyk2 LKP.KQFRKM IQQTFQQYAS LREEECVMKF FNTLAGFANI DQETYRCELI RatPyk2 LKP.KQFRKM IQQTFQQYAS LREEECVMKF FNTLAGFANI DQETYRCELI MousePyk2 LKP.KQFRKM IQQTFQQYAS LREEECVMKF FNTLAGFANI DQETYRCELI ChickenPyk2 LKP.KQFRKM IQQTFQQYAL LREEECILKF LHTLSTFAHI DQESYRCELI FrogPyk2 LKP.KQFRKM IQQTFQQYGS LKEEECITRF MNTLSTFTSI DQETYRCELA ZebrafishPyk21 MKQ.RQLRKL IQQTFQQYAI LKEDDCMVKF FETYGEFSNF DEEIFPCELV ZebrafishPyk22 MKP.KHLRKM IQQTFLQFAM LKEEQCIIKF FETLSVFSSF DEEVFPCELV FuguPyk2 MKS.KQLRKL IQQTFQQYAT LKEDECMVKF FETLNDFVSY DEEVFRCELV CionaFAK VKA.KDLRRM ITREFKTVGH LTEEQCVFRF FEILRKITRF DQEIFRCALG SeaUrchinFAK TKPKNLRKLI LQ.YFKQFAN LNEEQCVFKF FEVLTTVHRF DHEKFKCALG DrosophilaFAK SKPKNLKKL. IQVGYKKVYN YNDIEYLTRF FDLLKNIYLT NFEQFSVTLS AnophaeleFAK IKQ.KNLKKQ IQAGYKKVYS YSEMEYMLKF FDLLRTQYTF DQEQFNVQLS ApisFAK MKP.KALRKL IQQHFKKVAV LSELECMFKF FDLLRAHYRF DQERFICALG CelegansFAK FKH.SHLKKT FTAYIKKFSA TSPNESIIRS LALLLEVVKF DVELFKASLG Hydra WEEPRYQKNQ IQKLFKQYAN FKEDACCFKF YELLIAVQRF DLEVYKCSLG Hydractinia MKN.KDIRKK IQTLFKQYAD YKEDFCCFGF YKLLLSVHRF DLEVYKCSLG 351 400 HumanFAK SSWIISVELA IGPEEGISYL TDK.GCNPTH .LADFTQVQT IQYSNSE... ChimpFAK SSWIISVELA IGPEEGISYL TDK.GCNPTH .LADFTQVQT IQYSNSE... RatFAK SSWIISVELA IGPEEGISYL TDK.GCNPTH .LADFNQVQT IQYSNSE... MouseFAK SSWIISVELA IGPEEGISYL TDK.GCNPTH .LADFNQVQT IQYSNSE... ChickenFAK SSWIISVELA IGPEEGISYL TDK.GANPTH .LADFNQVQT IQYSNSE... FrogFAK SSWIISVELA IGPEEGISYL TDK.GSNPTH .LADFTQVQT IQYSSSE... Zebrafish1aFAK SSWVISVELA IGPEEGISYL TDK.GSNPTH .LANFTQVQT IQFE...... Zebrafish1bFAK SSWVISVDLA IGPEEGISYL TDK.GSTPTH .LANLTQVQS IHYSCVE... Fugu1newFAK SSWVIQVELA IGPEEGISYL TDK.GSTPTH .LANFNQVQS IQYSAME... Fugu2newFAK SSWIISVELA IGPEEGISYL TDK.GSTPTH .LANFNQVQS IQYSPLE... HumanPyk2 QGWNITVDLV IGPK.GIRQL TSQ.DAKPTC .LAEFKQIRS IRCLP..... ChimpPyk2 QGWNITVDLV IGPK.GIRQL TSQ.DAKPTC .LAEFKQIRS IRCLP..... RatPyk2 QGWNITVDLV IGPK.GIRQL TSQ.DTKPTC .LAEFKQIRS IRCLP..... MousePyk2 QGWNITVDLV IGPK.GIRQL TSQ.DTKPTC .LAEFKQIRS IRCLP..... ChickenPyk2 QGWNITVDLV IGPK.GIRQM TSK.EAKPTC .LAEFKHIKS IKCSS..... FrogPyk2 QGWSITVDLV IGPK.GIRQL TTK.DSKPTC .FAEFKQIKS IKCSL..... ZebrafishPyk21 QGWSLAVDLV IGPK.GIRQR TKA.DSTALC .LAEYKQIRS IKYNA..... ZebrafishPyk22 QGWSVSVDLV IGPK.GIRQR TDK.ISVPVS .LATFSQIRS IKCTH..... FuguPyk2 QGWSLAVELV IGGR.GIRQR TQK.NSAPVF .LADFKQIKK VQCWP..... CionaFAK TGWSVAVELV VGPEDGISYW TDK.GSSPTI .LAEFKHVQS IKTDSFT... SeaUrchinFAK TGWSISVELV IGPEDGISCL TDK.AATPTK .MADFDQVQS VQSLST.... DrosophilaFAK SAWNISGILH VGPHIGISYQ THP.QASLKN .VAQFKDVVS IKTCTLPKEK AnophaeleFAK SSWNIRVDLI IGPHVGLSYS VNP.QAPPTK VTDFESIERI TTSILPTDHQ ApisFAK SSWSIPVELV IGPDLGISYM AHR.GGTVPT RIAEFSQIQS IQTLVSD... CelegansFAK AGWTKPVELV VGPHTGLSYR LNE.RCDSSR .LLELRTIAE ITIRKME... Hydra SVWSIAVDVV VGPNDGISYR ..PEQGSKIN RMAEFFQISK IQSSKVG... Hydractinia SVWSIAVDVV IGPNDGISYR ..PEQGSKIT NMAHFKDIRS IYTSKVG... 401 450 HumanFAK ...DKDRKGM LQLKIAGAPE .......... ......PLTV TAPSLTIAEN ChimpFAK ...DKDRKGM LQLKIAGAPE .......... ......PLTV TAPSLTIAEN RatFAK ...DKDRKGM LQLKIAGAPE .......... ......PLTV TAPSLTIAEN MouseFAK ...DKDRKGM LQLKIAGAPE .......... ......PLTV TAPSLTIAEN ChickenFAK ...DKDRKGM LQLKIAGAPE .......... ......PLTV TAPSLTIAEN FrogFAK ...DKDRKGM LQLKIAGAPE .......... ......PLTV TAPSLTIAEN Zebrafish1aFAK ...ERERKGM LLLDVAGAPE .......... ......PLTV TTTTLNTAEN Zebrafish1bFAK ...EKERKGV LQLDVAGAAE .......... ......PLTI STPSFSTAEN Fugu1newFAK ...EKDRKGM IQLNVAGAAE .......... ......PLTV TTALLTTAEN Fugu2newFAK ...EKDRKGM LQLNVAGAPE .......... ......PLTV TTASLTTAEN HumanPyk2 ...LEEGQAV LQLGIEGAPQ .......... ......ALSI KTSSLAEAEN ChimpPyk2 ...LEEGQAV LQLGIEGAPQ .......... ......ALSI KTSSLAEAEN RatPyk2 ...LEETQAV LQLGIEGAPQ .......... ......SLSI KTSSLAEAEN MousePyk2 ...LEETQAV LQLGIEGAPQ .......... ......SLSI KTSSLAEAEN ChickenPyk2 ...VEEGRAV LQLGLSGTPQ .......... ......SLAI KTSSLAEAEN FrogPyk2 ...LEDGWAL LLLGISGAPQ .......... ......PLAI KTSSLAVAEN ZebrafishPyk21 ...QNDGRTL LFIEIDGAKQ .......... ......PLSV SVASLTIAEN ZebrafishPyk22 ...QNDGKAL LHINIEGAKQ .......... ......PLSI STASLALAEN FuguPyk2 ...QSDGKAL VHLDVEGARQ .......... ......RLSI NVPAVSVAEN CionaFAK ...DPNKKGT LLLKIQGAND .......... ......PLTI TTTSLYVAEN SeaUrchinFAK ...DNDKKGL LQLKIAGANE CDGNFHVYGH AAVDMDSVYI TTPSPTIAED DrosophilaFAK LSKSGENTTE PELQNFNCNC QKIKTQIKIS ASNNVEDLVI TCNGINTAES AnophaeleFAK GGRGAKGKDQ PDLTSSQCAC GEIKTQLRIR VNGNSEDLAI TCDGIKTSES ApisFAK ..CKEHAKAC IKLRIAGAAE .......... ......TLSI TCSSLDQAES CelegansFAK ...NGSEKTL MQLNLSGAAK .......... ......PVLI TLSTEELSQS Hydra ...AMPGKAS LTIQVEGSKE .......... ......PLVF MLDSINKAIE Hydractinia ...AMPGKAN LNIQVEGNNE .......... ......PLVF LVDPISKATE 451 500 HumanFAK MADLIDGYCR LVNGTSQSFI IRPQKEGE.. .......... .......... ChimpFAK MADLIDGYCR LVNGASQSFI IRPQKEGE.. .......... .......... RatFAK MADLIDGYCR LVNGATQSFI IRPQKEGE.. .......... .......... MouseFAK MADLIDGYCR LVNGATQSFI IRPQKEGE.. .......... .......... ChickenFAK MADLIDGYCR LVNGATQSFI IRPQKEGE.. .......... .......... FrogFAK MADLIDGYCR LVSGASESFI IRPQKEGE.. .......... .......... Zebrafish1aFAK MADLIDGYCR LVSGTSSSFI VRVQKEGE.. .......... .......... Zebrafish1bFAK MADLIDGYCR LLNAVNQSFI IRPQKDGE.. .......... .......... Fugu1newFAK LADLIDGYCR LISMESHSFI VRVHKEGE.. .......... .......... Fugu2newFAK MADLIDGYCR LVSSSPHSFI VRVHKGFR.. .......... .......... HumanPyk2 MADLIDGYCR LQGEHQGSLI IHPRKDGE.. .......... .........K ChimpPyk2 MADLIDGYCR LQGEHQGSLI IHLRKDGE.. .......... .........K RatPyk2 MADLIDGYCR LQGEHKGSLI IHAKKDGE.. .......... .........K MousePyk2 MADLIDGYCR LQGEHKGSLI MHAKKDGE.. .......... .........K ChickenPyk2 MADLIDGYCR LQGGLDTSLI VFPRREKE.. .......... .........K FrogPyk2 MADLIDGYCR LQNESKDSLI VNPKKDNE.. .......... .........K ZebrafishPyk21 MADLIDGYCR LFNHTDSSVI AGPNKNKS.. .......... .....QGVQL ZebrafishPyk22 MADLIDGYCR LEQGNEKTLI VWPNKVSM.. .......... .....SQIIY FuguPyk2 MMDLIDGYCR LERDTDETVI YRPNKGARGQ DCFLYQNKAK LLTCVPDASA CionaFAK IADLIDGYCR VVHGTDISFI VR........ .......... .....SQQYD SeaUrchinFAK MADLIDGYCR LVLNTEQSLI IRAQQ.AFNA VTRRSAKKAK NAVQDVIQGD DrosophilaFAK IADLIDGYCR LLSKDLEFTI .WHRE...TN ASNEDSAKAL PNDATLGSNK AnophaeleFAK IADLVDGYCR LFNKNDNS.L .WDRTTSSTD RHGSRSSSAD RLNGGSGGEL ApisFAK LADLIDGYCR LVTGTNTS.L .WNSS.RKDG HAFLYSLFAF LNSDNFDVDA CelegansFAK LAHLLDGYQM LYNQRD.S.V FKL.KGIERC ET........ .......... Hydra LADLIDGYCH IINGQLSKSL IV..KRVD.. .......... ........SY Hydractinia LADLIDGYCS IVSFHQPKSL II..KRAD.. .......... ........SY 501 550 HumanFAK .RALPSIPKL ANS....EKQ ......GMRT HAVSVS.... .......... ChimpFAK .RALPSIPKL ANS....EKQ ......GMRT HAFSVS.... .......... RatFAK .RALPSIPKL ANN....EKQ ......GMRT HAVSVS.... .......... MouseFAK .RALPSIPKL ANS....EKQ ......GMRT HAVSVS.... .......... ChickenFAK .RALPSIPKL ANN....EKQ ......GVRS HTVSVS.... .......... FrogFAK .RALPSIPKL ANN....EKH ......GVRP HAVSVS.... .......... Zebrafish1aFAK .RALPSIPKA PKS....ANH .EKRLQGVRA KAISIS.... .......... Zebrafish1bFAK .RALPSIPKL ANN....EKR LEGIRAGVR. .AISVS.... .......... Fugu1newFAK .RALPSIPKW THG....KRN ......GGWF LAVSAGL... .......... Fugu2newFAK .RIFVNDLQQ ILC....IAR ....RDHLLS PPFFSS.... .......... HumanPyk2 RNSLPQIPML NL.....EAR ........RS HLSESCSI.. .......... ChimpPyk2 RNSLPQIPML NL.....EAR ........RS HLSESCSI.. .......... RatPyk2 RNSLPQIPTL NL.....ESR ........RS HLSESCSI.. .......... MousePyk2 RNSLPQIPTL NL.....EAR ........RS HLSESCSI.. .......... ChickenPyk2 RSSLPRIPIP HL.....EER ........HS AVSDSLSV.. .......... FrogPyk2 RISLPQIPTE EV.....QVR ........RS RLVEGNSM.. .......... ZebrafishPyk21 RNSLPEIP.. .......SGK .........K TDSFRYS... .......... ZebrafishPyk22 WKLARRIPH. .......LTR .........K AKLSLFFA.. .......... FuguPyk2 RSSLPEIPS. .......GSS .........D RSSIRHSM.. .......... CionaFAK PRPLPSITLI NTDN...RKS FPIT...... .......... .......... SeaUrchinFAK RALPSLPGDS RLPSTEGSNE VTVSGRKKIP TVKPSG.... .......... DrosophilaFAK STSSQGKPM. .......... .......... .......... .......... AnophaeleFAK AEPPKPT... .......... .......... .......... .......... ApisFAK NLFSIFV... .......IVC RAKRFVYKYL APLYFYNIIP EKNVSKTGTI CelegansFAK .......... .......... .......... .......... .......... Hydra RN.LPSIPGE SSVA...IVP NRVSSSSLIL NSSKNGDF.. .......... Hydractinia RT.LPSVPKV AGSD..SSNT SSVSIINPRL SQGSLLYNNS KNG....... 551 600 HumanFAK ETDDYAEI.. IDEEDT.YTM P.......ST RDYEIQRERI ELGRCIGEGQ ChimpFAK ETDDYAEI.. IDEEDT.YTM P.......ST RDYEIQRERI ELGRCIGEGQ RatFAK ETDDYAEI.. IDEEDT.YTM P.......ST RDYEIQRERI ELGRCIGEGQ MouseFAK ETDDYAEI.. IDEEDT.YTM P.......ST RDYEIQRERI ELGRCIGEGQ ChickenFAK ETDDYAEI.. IDEEDT.YTM P.......ST RDYEIQRERI ELGRCIGEGQ FrogFAK ETDDYAEI.. IDEEDT.YTM P.......SA GDYEIQRDRI ELGRCIGEGQ Zebrafish1aFAK ETDDYAEI.. IDEEDT.YTM P.......ST RDYEIQRDRI ELGRCIGEGQ Zebrafish1bFAK ETDDYAEI.. IDEEDT.YTM P.......ST QDYEIQRDRI ELGRCIGEGQ Fugu1newFAK KTDDYAEI.. VDEEDT.YTM P.......ST RDYEIQRDRI ELGRCIGEGQ Fugu2newFAK ETDDYAEI.. IDEEDT.YTM P.......ST RDYEIQRDCI ELGRCIGEGQ HumanPyk2 ESDIYAEI.. PDETLRRPGG P......... .QYGIAREDV VLNRILGEGF ChimpPyk2 ESDIYAEI.. PDETLRRPGG P......... .QYGIAREDV VLNCILGEGF RatPyk2 ESDIYAEI.. PDETLRRPGG P......... .QYGVAREDV VLNRILGEGF MousePyk2 ESDIYAEI.. PDETLRRPGG P......... .QYGVAREEV VLNRILGEGF ChickenPyk2 DSEIYAEI.. PDETSRTRSG A......... QHYGVCRKDI TLGRILGEGF FrogPyk2 ESDIYAEI.. PEVTETKLTN N......... .RFTISRSDV ALGRILGEGF ZebrafishPyk21 NSDIYCEI.. PDEKP..PPP A......... .KVGLSRKDI SLKRMLGEGF ZebrafishPyk22 DSDIYAEI.. PEEKP..LAA C......... .KFSISRNDI VLGRILGEGF FuguPyk2 GSDIYCEI.. LDEKP..KSV E......... .KYGISRSDI VLGRILGAGF CionaFAK DTDDYAEI.. ..TEDM.YNV P.......ET FDYEIPRELI RIVTTVGEGQ SeaUrchinFAK LSDDYAEI.. VDDDD..YAM P.......AA KDYEISRELV TLVETIGQGQ DrosophilaFAK LTDDYAEIGL LEGEGD.YST P.......TV RNYELDRSLI TPSAKIGVGQ AnophaeleFAK LNEDYAELGM CDEEGD.YST P.......AA RDYELDRSQI TLNEIIGVGQ ApisFAK LSEDYAEI.. VDEEGD.YST PAKTLIIFAA RDYEIVRNQV ELGEIIGEGQ CelegansFAK LTMHEATI.. .RPKT...PN N.......ID SNIRLRRELI TLKELIGGGQ Hydra ..ADYAEI.. DDNSLRRDTN SN......SD KDFVVPYKNL TLIEIIGQGQ Hydractinia DFNDYAEI.A DDDDESDYAH PM......SA KDYEVARDDL TLDEIIGQGQ 601 650 HumanFAK FGDVHQGIYM SP........ .......... ........EN PALAVAIKTC ChimpFAK FGDVHQGIYM SP........ .......... ........EN PALAVAIKTC RatFAK FGDVHQGVYL SP........ .......... ........EN PALAVAIKTC MouseFAK FGDVHQGVYL SP........ .......... ........EN PALAVAIKTC ChickenFAK FGDVHQGIYM SP........ .......... ........EN PAMAVAIKTC FrogFAK FGDVHQGVYM SP........ .......... ........EN PAMAVAIKTC Zebrafish1aFAK FGDVHQGIYI CP........ .......... ........DT PALSVAIKTC Zebrafish1bFAK FGDVHQGVYI SP........ .......... ........EN PSLSVAIKTC Fugu1newFAK FGDVHQGVYN SP........ .......... ........DK PALHVAIKTC Fugu2newFAK FGDVHQGLYI SP........ .......... ........EN PALSVAVKTC HumanPyk2 FGEVYEGVYT NH........ .......... ........KG EKINVAVKTC ChimpPyk2 FGEVYEGVYT NH........ .......... ........KG EKINVAVKTC RatPyk2 FGEVYEGVYT NH........ .......... ........KG EKINVAVKTC MousePyk2 FGEVYEGVYT NH........ .......... ........KG EKINVAVKTC ChickenPyk2 FGEVYEGTYT NP........ .......... ........KG ERVNVAVKTC FrogPyk2 FGEVYDGVYV KP........ .......... ........KG DKINVAVKTC ZebrafishPyk21 FGEVHEGVYQ KK........ .......... ........NG EKVNVAVKTC ZebrafishPyk22 FGEVHDGIYK SK........ .......... ........SG ERVNVAVKTC FuguPyk2 FGEVHEGVYK S......... .......... ........SG ENISVAVKTC CionaFAK FGDVHKGIYS TR........ .......... ........ET PDLQVAIKTC SeaUrchinFAK FGDVHKGIYI D......... .......... ........QD EKVEVAVKTC DrosophilaFAK FGDVYVGTYT LP..KLGKGK NLAGNGKNSN SDQRNADSRP DVIQVAIKTC AnophaeleFAK FGDVHIGSCR LPNNKLNQSL TSEFDEYSQM DNGNADAQKT GIIQVAVKTC ApisFAK FGNVHKGSYK GR........ .......... ........DN QVIPVAVKTC CelegansFAK FGNVYKAVYH DLE....... .......... ........KD ERIAVAVKVC Hydra FGDVYRGICK TT........ .......... ........LN SELPVAVKTY Hydractinia FGDVYQGTFK SKI....... .......... ........SS VEIPVAVKTY 651 700 HumanFAK KN....CTSD S..VREKFLQ EALTMRQFDH PHIVKLIGVI TENPVWIIME ChimpFAK KN....CTSD S..VREKFLQ EALTMRQFDH PHIVKLIGVI TENPVWIIME RatFAK KN....CTSD S..VREKFLQ EALTMRQFDH PHIVKLIGVI TENPVWIIME MouseFAK KN....CTSD S..VREKFLQ EALTMRQFDH PHIVKLIGVI TENPVWIIME ChickenFAK KN....CTSD S..VREKFLQ EALTMRQFDH PHIVKLIGVI TENPVWIIME FrogFAK KN....CTSD S..VREKFLQ EALTMRQFDH PHIVKLIGVI TENPVWIIME Zebrafish1aFAK KN....CTSD S..VREKFLQ EALTMRQFDH PHIVKLIGVI TENPVWIIME Zebrafish1bFAK KN....CTSD S..VREKFLQ EALTMRQFDH PHIVKLIGII TENPVWIIME Fugu1newFAK KN....CTSD S..VREKFLQ EALTMRQFDH PHIVKLIGVI TENPVWIIME Fugu2newFAK KN....STSD S..VREKFLQ EALTMRQFDH PHIVKLMGVI TENPVWIIME HumanPyk2 KK...DCTLD ...NKEKFMS EAVIMKNLDH PHIVKLIGII EEEPTWIIME ChimpPyk2 KK...DCTLD ...NKEKFMS EAVIMKNLDH PHIVKLIGII EEEPTWIIME RatPyk2 KK...DCTLD ...NKEKFMS EAVIMKNLDH PHIVKLIGII EEEPTWIVME MousePyk2 KK...DCTQD ...NKEKFMS EAVIMKNLDH PHIVKLIGII EEEPTWIIME ChickenPyk2 KK...DCSPE ...NKDKFLS EARLMKNLDH PHIVKLIGIA EEEPTWIIME FrogPyk2 KN...DCAAD ...VKEKFMS EAYLMRNLDH PHIVKLIGII EDDPIWIVME ZebrafishPyk21 K....ECAPD ...IREKFVG EAVIMKNLDH PHIVRLIGVI EEDPVWIVME ZebrafishPyk22 K....DCSAD ...VKEKFMS EALIMKKLDH PHIVRLIGII EEDPVWIVME FuguPyk2 K....DCSPD ...VMEKFMS EAVIMKKLNH QHIVRLIGII EENPVWIVME CionaFAK KF....QGQD GMGVADRLLE EAHTMRQFEH PHIVKLIGVC TEEPVWIVME SeaUrchinFAK KV.....EST Q.ALGERFLE EAYIMKQFDH PHIIKLIGVC TDPPIWIVME DrosophilaFAK KA.....NDD P.EKTENFLA EAYIMQKFDH PHIIRLIGIC SVMPIWIVME AnophaeleFAK KP.....DAD T.TTSEKFLQ EAYIMKKFEH PHIIKLIGIS SGPPIWIVME ApisFAK KV.....DAD L.ATSEKFLE EAYIMQQFEH PHIIRLIGVC SEPPIWLVME CelegansFAK KT.....DAE P.ADTQLILQ ESSLMRNFRH SNIIQLIGVC VDQPMWLVLE Hydra KVELEKEQED A.TRAEKFLE EAYIMKLFDH PHIIKLIGIC PENPPYIIME Hydractinia KIELDKEQED G.TRAEKFLE EAYLMQQFDH PHVIKLIGIV SDSPSYIIME 701 750 HumanFAK LCTLGELRSF LQVRKYSLDL ASLILYAYQL STALAYLESK RFVHR..DIA ChimpFAK LCTLGELRSF LQVRKYSLDL ASLILYAYQL STALAYLESK RFVHR..DIA RatFAK LCTLGELRSF LQVRKYSLDL ASLILYAYQL STALAYLESK RFVHR..DIA MouseFAK LCTLGELRSF LQVRKYSLDL ASLILYAYQL STALAYLESK RFVHR..DIA ChickenFAK LCTLGELRSF LQVRKFSLDL ASLILYAYQL STALAYLESK RFVHR..DIA FrogFAK LCTLGELRSF LQVRKYSLDL ASLILYSYQL STALAYLESK RFVHR..DIA Zebrafish1aFAK LCTLGELRSF LQIRKYNLDL ASLILFSYQL STALAYLESK RFVHR..DIA Zebrafish1bFAK LCTLGELRSF LQVRKYNLDL STLILFAYQL STALAYLESK RFVHR..DIA Fugu1newFAK LCTLGELRSF LQVRKYSLDL ATLILFAYQL STALAYLESK RFVHR..DIA Fugu2newFAK LCTLGELRSF LQVRKYSLDL ATLILYSYQL STALAYLESK RFVHR..DIA HumanPyk2 LYPYGELGHY LERNKNSLKV LTLVLYSLQI CKAMAYLESI NCVHR..DIA ChimpPyk2 LYPYGELGHY LERNKNSLKV LTLVLYSLQI CKAMAYLESI NCVHR..DIA RatPyk2 LYPYGELGHY LERNKNSLKV PTLVLYALQI CKAMAYLESI NCVHR..DIA MousePyk2 LYPYGELGHY LERNKNSLKV PTLVLYTLQI CKAMAYLESI NCVHR..DIA ChickenPyk2 LYPYGEVGQY LEQNKHCLAV PTLVLYALQI SKALAYLEAI NCVHR..DIA FrogPyk2 LYPYGELGQY LEKNKNSLQV LSLILYSLQI CKALAYLEGI NCVHR..DIA ZebrafishPyk21 LCQQGELGKY LTKNQQSLTS VTLVLFSLQL CKALVYLQGM NMVHR..DIA ZebrafishPyk22 LYPFGELENY LKDNQNKLTN VTLILYSLQI SKALAYLEGV NMVHR..DIA FuguPyk2 LYQYGELGNY LVKNQSTLTN TTLLLFSLQI CKALVYLEGV SMVHR..DIA CionaFAK FCKYGEMRSY LQTNKHNLDV TMLITYACQL SEALCYLEHK KFVHR..DIA SeaUrchinFAK LAPFGEMRMF LQTHKEELTL ASLILYAYQL SMALSYLESK KFVHR..DIA DrosophilaFAK LAKLGELRAY LKTNSERLSH GTLLKYCYQL STALSYLESK KFVHR..DIA AnophaeleFAK LARHGELRAY LKKNGPKLKL GTLLLYSYQL STALSYLESK KFVHR..DIA ApisFAK LAKLGEMRAY LQSNKHRLDL ATLLLYTFQL STALSYLESK KFVHRYRDIA CelegansFAK LAPKGELREY LQQEKDWLPL RILTLFCSQI CDSLVYLHST RFVHR..DIA Hydra LAVYGELRSY LQTYKIQTEL ERLIAYIYQL STALCYLESK NFVHR..DVA Hydractinia LASYGELRSY LQQYRTQTES ERLLTYIYQL STALSYLESR NFVHR..DIA 751 800 HumanFAK ARNVLVSSND CVKLGDFGLS RYMED.STYY K......... .......... ChimpFAK ARNVLVSSND CVKLGDFGLS RYMED.STYY K......... .......... RatFAK ARNVLVSSND CVKLGDFGLS RYMED.STYY K......... .......... MouseFAK ARNVLVSSND CVKLGDFGLS RYMED.STYY K......... .......... ChickenFAK ARNVLVSATD CVKLGDFGLS RYMED.STYY K......... .......... FrogFAK ARNVLVSSSD CVKLGDFGLS RYMED.STYY K......... .......... Zebrafish1aFAK ARNVLVSSVD CVKLGDFGLS RYMED.SSYY K......... .......... Zebrafish1bFAK ARNVLVSSID CVKLGDFGLS RYMED.SSYY K......... .......... Fugu1newFAK ARNVLVSSVD CVMLGDFGLS RYMED.SSYY K......... .......... Fugu2newFAK ARNVLVSTVD CVKLGDFGLS RYMED.SSYY K......... .......... HumanPyk2 VRNILVASPE CVKLGDFGLS RYIEDED.YY K......... .......... ChimpPyk2 VRNILVASPE CVKLGDFGLS RYIEDED.YY K......... .......... RatPyk2 VRNILVASPE CVKLGDFGLS RYIEDED.YY K......... .......... MousePyk2 VRNILVASPE CVKLGDFGLS RYIEDED.YY K......... .......... ChickenPyk2 VRNILVASPE CVKLGDFGLS RYIEDEE.YY K......... .......... FrogPyk2 VRNILVASPE CVKLGDFGLS RYLEEEE.YY K......... .......... ZebrafishPyk21 VRNVLVATPE CVKLGDFGLS RYIEDEE.YY K......... .......... ZebrafishPyk22 VRNVLVAKVD CVKLGDFGLS RYIEEEE.YY K......... .......... FuguPyk2 VRNILVAAPD CVKLGDFGLS RYIEDEE.YY KGRRSAFGAT RLRYSFGEEI CionaFAK ARNILVAQPD CIKLGDFGLS RYMED.QNYY T......... .......... SeaUrchinFAK ARNLLVVAKD NVKLGDFGLS RLLHD.HSYY K......... .......... DrosophilaFAK ARNVLVSSPT CVKLADFGLS RWVSD.QSYY H......... .......... AnophaeleFAK ARNVLVSSPT CIKLADFGLS RWVED.QSYY T......... .......... ApisFAK ARNVLVSAHN SVKLADFGLS RWVED.QSYY T......... .......... CelegansFAK ARNILVCSPQ CVKLADFGLS RALDY.DAVY T......... .......... Hydra ARNILVVDPL TVKLADFGLS RWIDEEQLYY K......... .......... Hydractinia ARNILAADPH TIKLADFGLS RWIDEEQSYY K......... .......... 801 850 HumanFAK .......... ....ASKGKL PIKWMAPESI NFRRFTSASD VWMFGVCMWE ChimpFAK .......... ....ASKGKL PIKWMAPESI NFRRFTSASD VWMFGVCMWE RatFAK .......... ....ASKGKL PIKWMAPESI NFRRFTSASD VWMFGVCMWE MouseFAK .......... ....ASKGKL PIKWMAPESI NFRRFTSASD VWMFGVCMWE ChickenFAK .......... ....ASKGKL PIKWMAPESI NFRRFTSASD VWMFGVCMWE FrogFAK .......... ....ASKGKL PIKWMAPESI NFRRFTSASD VWMFGVCMWE Zebrafish1aFAK .......... ....ASKGKL PIKWMAPESI NFRRFTSASD VWMFGVCMWE Zebrafish1bFAK .......... ....ASKGKL PIKWMAPESI NFRRFTSASD VWMFGVCMWE Fugu1newFAK .......... ....ASKGKL PIKWMAPESI NFRRFTSASD VWMFGVCMWE Fugu2newFAK .......... ....ASKGKL PIKWMAPESI NFRRFTTASD VWMFGVCMWE HumanPyk2 .......... ....ASVTRL PIKWMSPESI NFRRFTTASD VWMFAVCMWE ChimpPyk2 .......... ....ASVTRL PIKWMSPESI NFRRFTTASD VWMFAVCMWE RatPyk2 .......... ....ASVTRL PIKWMSPESI NFRRFTTASD VWMFAVCMWE MousePyk2 .......... ....ASVTRL PIKWMSPESI NFRRFTTASD VWMFAVCMWE ChickenPyk2 .......... ....ASVTRL PIKWMSPESI NFRRFTTASD VWMFAVCMWE FrogPyk2 .......... ....ASVTRL PIKWMAPESI NFRRFTSASD VWMFGVCMWE ZebrafishPyk21 .......... ....ASVTRL PIKWMAPESI NFRRFTAASD VWMFAVCVWE ZebrafishPyk22 .......... ....ASVTRL PIKWMAPESI NFRRFTSASD VWMFAVCMWE FuguPyk2 RRSAGCVFIC YFSAASVTRL PIKWMAPESI NFRRFTTASD VWMFAVCMWE CionaFAK .......... ....ASKGKL PIKWMAPESI NFRRFTTATD VWMFAVCCWE SeaUrchinFAK .......... ....ASKGKL PIKWMAPESI NFRRFTSASD VWMFGVCMWE DrosophilaFAK .......... ...STPTVAL PIKWMSPESI NFRRFTTASD VWMFGVCIWE AnophaeleFAK .......... ....STKGML PIKWMAPESI NFRRFTTASD VWMFGVCTWE ApisFAK .......... ....ASRCKL PIKWMAPESI NFRRFTTSSD VWMFGVCMWE CelegansFAK .......... ....ASRGKL PIKWLAPESV NYRQFSMASD VWMFGVCMWE Hydra .......... ....ASKGKL PIKWMAPESI NFRRFSSKSD VWMFGVCCWE Hydractinia .......... ....ASKGKL PIKWMAPESI NFRRFSSASD VWMFGVCCWE 851 900 HumanFAK ILMHGVKPFQ GVKNNDVIGR IENGERLPMP PNCPPTLYSL M.TKCWAYDP ChimpFAK ILMHGVKPFQ GVKNNDVIGR IENGERLPMP PNCPPTLYSL M.TKCWAYDP RatFAK ILMHGVKPFQ GVKNNDVIGR IENGERLPMP PNCPPTLYSL M.TKCWAYDP MouseFAK ILMHGVKPFQ GVKNNDVIGR IENGERLPMP PNCPPTLYSL M.TKCWAYDP ChickenFAK ILMHGVKPFQ GVKNNDVIGR IENGERLPMP PNCPPTLYSL M.TKCWAYDP FrogFAK ILMYGVKPFQ GVKNNDVIGR IENGERLPMP PNCPPTLYSL M.TKCWAYDP Zebrafish1aFAK ILMYGVKPFQ GVKNNDVIGR IENGERLAMP PNCPPTLYSL M.TKCWAYDP Zebrafish1bFAK ILMFGIKPFQ GVKNNDVIGR IENGERLAMP QNCPPTLYSL M.TKCWAYDP Fugu1newFAK ILMYGIKPFQ GVKNNDVIGR IENGERLAMP PQCPPTLYSL M.TKCWSYDP Fugu2newFAK ILMFGIKPFQ GVKNNDVIGR IENGERLAMP PQCPPTLYSL M.TKCWSYDP HumanPyk2 ILSFGKQPFF WLENKDVIGV LEKGDRLPKP DLCPPVLYTL M.TRCWDYDP ChimpPyk2 ILSFGKQPFF WLENKDVIGV LEKGDRLPKP DLCPPVLYTL M.TRCWDYDP RatPyk2 ILSFGKQPFF WLENKDVIGV LEKGDRLPKP ELCPPVLYTL M.TRCWDYDP MousePyk2 ILSFGKQPFF WLENKDVIGV LEKGDRLPKP ELCPPVLYTL M.TRCWDYDP ChickenPyk2 ILSYGRQPFF WLENKDVIGV LERGDRLPKP DLCPPILYTL M.TRCWDYDP FrogPyk2 ILSFGKQPFF WLENKDVISV IEKGDRLPKP EMCPPTLYTL M.TRCWTYDP ZebrafishPyk21 IMNRGKQPFH WLDNRDVINQ LEQGNRLPKP EQCPPALYSL M.TRCWSYDP ZebrafishPyk22 IMSGGQQPFF WLENKDVINQ LEQGMRLPKP ERCPPTLYSL M.TRCWSYSP FuguPyk2 IMSYGQQPFF WLENRDVINQ LEHGIRLPKP ETCPPALYSL M.TRCWAYDP CionaFAK ILMMGVKPFQ GVKNNDVIGK IEAGERLAMP SNCQPALYDL M.LRCWSYDP SeaUrchinFAK ILMYGVKPFQ GVKNNEVIGR IENGERLPMP AACPPTLYSV M.TLCWSYEP DrosophilaFAK ILMLGVKPFQ GVKNSDVILK LENGERLPLP PNCPPRLYSL M.SQCWAYEP AnophaeleFAK ILMLGIKPFQ GVKNCDVIGK LENGERLPLP PNCPPRLYSL M.SQCWSLEP ApisFAK ILMLGVKPFQ GVKNNEVIRK LENGERLALP NHCPPRLYSL M.SQCWSYEP CelegansFAK IFSLGVKPWA GVTNSDVIMH IEQGSRPPCP EKCPTALYNF IRSKMWAIEP Hydra ILMYGVKPFQ GVPNEKVIGK IENGERLQLP PNCPPTLYHI M.TETWLYDP Hydractinia ILMYGVKPFQ GVPNEKVIGK IENGERLPLP PNCAPTLYHL M.TESWHYEP 901 950 HumanFAK SRRPRFTELK AQLSTILEEE KAQQEERMRM ESRRQATVSW DSGGSDE..A ChimpFAK SRRPRFTELK AQLSTILEEE KAQQEERMRM ESRRQATVSW DSGGSDE..A RatFAK SRRPRFTELK AQLSTILEEE KVQQEERMRM ESRRQATVSW DSGGSDE..A MouseFAK SRRPRFTELK AQLSTILEEE KVQQEERMRM ESRRQATVSW DSGGSDE..A ChickenFAK SRRPRFTELK AQLSTILEEE KLQQEERMRM ESRRQVTVSW DSGGSDE..A FrogFAK SRRPRFTELK AQLSTILEEE KLQQEERMRM ESRRQVTVSW DSGGSDE..A Zebrafish1aFAK SKRPRFTELK GQLSTILAEE KAQQEERSRM EMRRQVPVSW DSGGSDE..A Zebrafish1bFAK SKRPRFTELK TQLSTILDEE KLQQEERFRM EMRRQVTVSW DAGNSDE..A Fugu1newFAK SKRPRFTELK TQLSTILEEE KLQEEERHRM EMRRQVNMSW DSGGSDE..A Fugu2newFAK SKRPRFTELK TQLSTILEEE KLQQKERNRM EMRRQVTVSW DSGGSDE..A HumanPyk2 SDRPRFTELV CSLSDVYQME KDIAMEQERN ARYRTPKILE PTAFQ.E..P ChimpPyk2 SDRPRFTELV CSLSDVYQME KDIAMEQERN ARYRTPKILE PTAFQ.E..P RatPyk2 SDRPRFTELV CSLSDIYQME RDIAIEQERN ARYRPPKILE PTAFQ.E..P MousePyk2 SDRPRFTELV CSLSDIYQME KDIAIEQERN ARYRPPKILE PTTFQ.E..P ChickenPyk2 NERPKFKDLV CSLSDIYLME KNLANEQERN NRHRPPKIME PPTPQ.E..P FrogPyk2 FERPKFTELV CSLSDIYQTE KEIIKDKERN NRQRPPKIIE PPPMQ.E..P ZebrafishPyk21 RERPTFTELA CNISDVHQME KELEAERERD KARNIRTL.E PKINISE..P ZebrafishPyk22 RDRPSFTELV CKLSDVHKLE QEVEVEELR. ARSRTINISA FP...D...A FuguPyk2 GERPRFTELV LKI....... .......... ...RTSCNT. .........R CionaFAK GFRPLFEEIQ HELYIILDDV THQQDELRRL ESGG.VAAQW H.GA.DE..P SeaUrchinFAK SKRPNFKDLK TRLNEILEEE KEQEEAALKQ EKRRILST.W ..GF.PE..E DrosophilaFAK LKRPNFKRIK ETLHEILIED SINSSETLKR EQRKVASMSW I.GSDDIDIP AnophaeleFAK LKRPNFKSVK ETLYEILMEE RHSDCETMRR ENRRVAAMSW ..GAGDDM.A ApisFAK SKRPTFKEIR ETLHEILLEE KHQQQETMRR ENRRVQAMSW VVGA.DDV.P CelegansFAK HKRPTVDQIY AIIEDVR... ..QQIIQN.. .......... .........I Hydra EKRPTFQQMK LRLSAIAEEE KNSVLERKKK EERRMHAKPP SNEN.....A Hydractinia SKRPTFQELK SRLSQIKYEE SFSGQERKKK EDRRIKMNSF ..GS.DEAFA 951 1000 HumanFAK PPKPSRPGYP SPRSSEGFYP .....SPQHM VQTNHYQVSG YPGSHGI... ChimpFAK PPKPSRPGYP SPRSSEGFYP .....SPQHM VQTNHYQVSG YPGSHGI... RatFAK PPKPSRPGYP SPRSSEGFYP .....SPQHM VQTNHYQISG YPGSHGI... MouseFAK PPKPSRPGYP SPRSSEGFYP .....SPQHM VQTNHYQVSG YPGSHGI... ChickenFAK PPKPSRPGYP SPRSSEGFYP .....SPQHM VQPNHYQVSG YSGSHGI... FrogFAK PPKPSRPGYP SPRSSEGFFP .....SPQHM MQPNHYQVSG FSVAHGI... Zebrafish1aFAK PPKPSRPGYP SPRSSEGFYP .....SPQHM GQHNHYQMAG HSGPHGL... Zebrafish1bFAK PPKPSRPGYP SPRSSDGYFP .....SPQHA GQHNHYQGSG YPAAHVS... Fugu1newFAK PPKPSRPGYP SPRSSEGFFP .....SPQL. ...NHFQPSA HAGGGGG... Fugu2newFAK PPKPSRPGYP SPRSSEGFYP .....SSQH. ..PGHYQMAG YPGPHTL... HumanPyk2 PPKPSRPKYR PPPQTNLLAP .....KLQFQ VPEGLCASSP TLTSPME... ChimpPyk2 PPKPSRPLYR PPPQTNLLAP ......MQFQ VPEGLCASSP TLTSPME... RatPyk2 PPKPSRPKYK HPPQTNLLAP .....KLQFQ VPEGLCASSP TLTSPME... MousePyk2 PPKPSRPKYR PPPQTNLLAP .....KLQFQ VPEGLCASSP TLTSPME... ChickenPyk2 PPKPSRPRYK PPPQSNLLAP .....KLQFQ VPEGLCASSP TLTSPAE... FrogPyk2 PPKPSRPKYK AAAPGNLLAP .....NLLTQ VPESLCASSP TLCSPTD... ZebrafishPyk21 PPKPSRVN.. TTRFGNTLGV GL...HLQLN ..EALCASSP DLASPCD... ZebrafishPyk22 PPKPSRMI.. .PPGFNTLSP RL...NVQLH ..SKALLYFD ILV....... FuguPyk2 PQP.SRMK.. PGRFGNTLSI GL...HIQLN ..EALCASSP DLASPS.... CionaFAK PPKPSRPGFP GSQGARGYPP QP...GAQGP ..LYQPTNQG YLGGR..... SeaUrchinFAK PPPKERTEQE KQNELE.... .......... .......... .......... DrosophilaFAK PSKPSRVMHD PDITGLMPET TG...LPQTY IIAQNPAVLA KLMMENQK.. AnophaeleFAK PPKPARGPTM GDGPLVPGA. ......PQTY IVARDPTVLA ALMRENEQ.. ApisFAK PPKPSRQPQN TTDQSQLTA. ...AAPVSTY IVAQNPEVLA QLLKDNQT.. CelegansFAK PPEQIIVGKP MTAAGVIVAE M..SSLPGLT .......... .......... Hydra PPK....... .......... .......... .......... .......... Hydractinia PPKPARPTGR IEPLFLNKSQ TLPRNMRQSP ETALTSMSTS TLPRPVTKNE 1001 1050 HumanFAK .TAMAGSIYP GQASLL..DQ TDSWNHRPQE I......... .......... ChimpFAK .TAMAGSIYP GQASLL..DQ TDSWNHRPQE I......... .......... RatFAK .PAMAGSIYP GQASLL..DQ TELWNHRPQE M......... .......... MouseFAK .PAMAGSIYQ GQASLL..DQ TELWNHRPQE M......... .......... ChickenFAK .PAMAGSIYP GQASLL..DQ TDSWNHRPQE V......... .......... FrogFAK .PSMSGNMYP GQASVL..DH MDSWNHRTPD I......... .......... Zebrafish1aFAK .LSVPGGLYP PQATVTGLDP HDSWNHRRVA QD........ .......... Zebrafish1bFAK ...SPG.... FPAQTSILDP LESWNHR.QE P......... .......... Fugu1newFAK ...VGGSF.. ........TP TDSWSQHRSE HT........ .......... Fugu2newFAK .PSVPSALYP PQPTMVDTHH AHTHPRHHVH AHSHAQHLPH EM........ HumanPyk2 ........YP SPVNSLHTPP ..LHRHNVFK RH.SMREEDF IQPSSREEAQ ChimpPyk2 ........YP SPVNSLHTPP ..LHRHNVFK RH.SMREEDF IRPSSREEAQ RatPyk2 ........YP SPVNSLHTPP ..LHRHNVFK RH.SMREEDF IRPSSREEAQ MousePyk2 ........YP SPVNSLHTPP ..LHRHNVFK RH.SMREEDF IRPSSREEAQ ChickenPyk2 ........YQ SPASSLHTPP ..LHRHNVFK RH.SMREEDF LRPSSREEAQ FrogPyk2 ........YQ SPLDSTRSPP ..LPRHSIFK RR.SMREEDF VKPNSKEEAQ ZebrafishPyk21 ........YQ SPVDSTNLLS ..LPKTPNRR LSLGEGDFS. FGPSSKEDAQ ZebrafishPyk22 ........YP SMHKTHGDLC V....CLSLS QSLSLCVGV. QEPSSKADAQ FuguPyk2 ........YQ SPLDSMNVLP ..LPVRSPRR RSVGEGEFLR VEATSKEDAQ CionaFAK .PAIPPKSVP RIMTAEELAN HEAQEK.... .......... .......... SeaUrchinFAK .......... .......... .......... .......... .......... DrosophilaFAK .RGINPAAYT TPASGIHN.. .......... .......... .......... AnophaeleFAK .RGINPSSYT TPASVFN... .......... .......... .......... ApisFAK .RGVCPSVYT TPASPFN... .......... .......... .......... CelegansFAK .......... .......... .......... .......... .......... Hydra .......... .......... .......... .......... .......... Hydractinia SPFIVQRLS. .......... ...WSGARNH ARQEKQ...K READDKAKLI 1051 1100 HumanFAK AMWQPNVEDS TVLDLRGIGQ VLPTHLMEER LIRQQQEMEE DQRWLEKEE. ChimpFAK AMWQPNVEDS TVLDLRGIGQ VLPTHLMEER LIRQQQEMEE DQRWLEKEE. RatFAK SMWQPSVEDS AALDLRGMGQ VLPPHLMEER LIRQQQEMEE DQRWLEKEE. MouseFAK SMWQPSVEDS AALDLRGMGQ VLPPHLMEER LIRQQQEMEE DQRWLEKEE. ChickenFAK SAWQPNMEDS GTLDVRGMGQ VLPTHLMEER LIRQQQEMEE DQRWLEKEE. FrogFAK NMWQPSMEDS GPMDMRSLAQ VLPTHLMEER LIRQQQEMEE DQRWLEKEE. Zebrafish1aFAK HMWNPSMEDG VALRGGL.SQ .....LMEEQ LILQQQQMED DQRWLEHEE. Zebrafish1bFAK PTWPANAEDG AAV...GRTQ PMNTHLMEET LIKQQQQMEE DQRWLEQEE. Fugu1newFAK AMWSPNIEHQ CSVQ..GNGQ A....QMEER LMMQQQQMED DQRWLEQEE. Fugu2newFAK ALWGPGIECL .......... .....FMEEQ LMMQQQQMEE DQRWLEQEE. HumanPyk2 QLWEAEKV.. .......... .....KMRQI LDKQQKQMVE DYQWLRQEE. ChimpPyk2 QLWEAEKV.. .......... .....KMRQI LDKQQKQMVE DYQWLRQEE. RatPyk2 QLWEAEKI.. .......... .....KMRQV LDRQQKQMVE DSQWLRREE. MousePyk2 QLWEAEKI.. .......... .....KMKQV LERQQKQMVE DSQWLRREE. ChickenPyk2 KLWEMERL.. .......... .....KMRQV LDKQQKQMVE DYQWLRQEE. FrogPyk2 KLWEKERD.. .......... .....KMLQV LNRQQQQMEE DGKWLKQEE. ZebrafishPyk21 RLWQMEKD.. .......... .....RLQDT LKMQKEQMEE DTKWLKKEE. ZebrafishPyk22 RLWDVERQ.. .......... .....QVQET LRRQKQEMLE DHQWLEKEE. FuguPyk2 RLWQRERM.. .......... .....QMQET LRRQKEQMME DNKWLAKEE. CionaFAK .......... ....LRQRQF EDS..KLEEK LMIQKKQAEA DKLWLQQEEE SeaUrchinFAK .......... .......... .....RIGDA LRKQRLESEA DSEWLATSAQ DrosophilaFAK .......... .......... .....VLGEK LRQQQKDSNS DSEWLIQEEL AnophaeleFAK .......... .......... .....TLAEK LRKQQQESEI DSKWLQQEEN ApisFAK .......... .......... .....TLAVQ FQDEDQESAS PPVPRSVTQS CelegansFAK .......... .......... ......LYRT MEDQKRQAEE DAKWLEQEDD Hydra .......... .......... .......... .......... .......... Hydractinia RKTRAE.... .......... QES...LRRK LNEQKEQSDA DSQWLMSEEV 1101 1150 HumanFAK .RFL...... ...KPDVRLS RG........ SIDREDGSLQ GPIGNQHIYQ ChimpFAK .RFL...... ...KPDVRLS RG........ SIDREDGSLQ GPIGNQHIYQ RatFAK .RFL...... ...KPDVRLS RG........ SIDREDGSFQ GPTGNQHIYQ MouseFAK .RFL...... ...KPDVRLS RG........ SIDREDGSFQ GPTGNQHIYQ ChickenFAK .RFLVM.... ...KPDVRLS RG........ SIEREDGGLQ GPAGNQHIYQ FrogFAK .RFL...... ...KPDVRLS RG........ SVDHVDGNIQ CPAGNQHIYQ Zebrafish1aFAK .RLL...... ...KPDGRSS RG........ STERDDGSLQ GPTGNQHIYQ Zebrafish1bFAK .SFL...... ...KPESRNS RS........ SIDREDAALQ TPMGKQHVYQ Fugu1newFAK .SFM...... ...KTESRNS RG........ SIDREDGTLQ APVSLCVHVR Fugu2newFAK .RLLVTHTLE KKKNYKSRGS RG........ SLDREDGVLQ PPTGNQHIYQ HumanPyk2 .KSL...... .......... .......... ......DPMV YMNDKSPLTP ChimpPyk2 .KSL...... .......... .......... ......DPMV YMNDKSPLTP RatPyk2 .RCL...... .......... .......... ......DPMV YMNDKSPLTP MousePyk2 .RCL...... .......... .......... ......DPMV YMNDKSPLTP ChickenPyk2 .KSL...... .......... .......... ..AGKTMKVS RALFQQSSPG FrogPyk2 .KSL...... .......... .........A NTLKDSKQKT NNNKPPDALP ZebrafishPyk21 .KLL...... .......... .......... ......DPMV HEGSKIPEPP ZebrafishPyk22 .KLL...... ......SSNS .......... ........VA DADMFVVISP FuguPyk2 .RLLVGSRAT LTTLSPLCVS VCPPASTFSA ALGATPSGES LSHFQPPDRS CionaFAK QLKPERTPSN .......... .........G DLEEQDP... ..DHLYQRRN SeaUrchinFAK TLRPDVSRTE GNIFLQEGQN GNREPYGGAS QPLYEAFNQK SSSAPSTMTT DrosophilaFAK LRQRSCSIPQ GSLNDHQAQM FKLDFMSAGP SSLPDCSNSS SRPMTPNANL AnophaeleFAK NLKKRLSLIT ANAASMSLDQ PLGGGAPMEG STPPVNGGGS SLPHTPGSGG ApisFAK PSHEQHGANT GSIGSDKSTE KVIVVKKMEP T......... .......... CelegansFAK EDEDDQDIDQ IPSTSHSSVE NIRT...... .......... .......... Hydra .......... .......... .......... .......... .......... Hydractinia ASYAEVQPSA DDQNFQRHSW KDGLHPSGSK SDSEQLGYDL FKEDFARDII 1151 1200 HumanFAK PVGKPDPAA. .PPKKPPRPG APGHLGSLAS LSSPADSYNE GVK.LQPQEI ChimpFAK PVGKPDPAA. .PPKKPPRPG APGHLGSLAS LSSPADSYNE GVK.LQPQEI RatFAK PVGKPDPAA. .PPKKPPRPG APGHLSNLSS ISSPAESYNE GVK.LQPQEI MouseFAK PVGKPDPAA. .PPKKPPRPG APGHLSNLSS ISSPADSYNE GVK.LQPQEI ChickenFAK PVGKPDHAA. .PPKKPPRPG AP.HLGSLAS LNSPVDSYNE GVK.IKPQEI FrogFAK PVGKPDHVA. .PPKKPPRPG APSHLGNLPA HNSPVDGYNE GVK.IQPQEI Zebrafish1aFAK PIGKAEHAA. .PPKKPPRPG APSHAAGVSG LN.PTESYNE GVK.IQPQEI Zebrafish1bFAK PVGKTDLVA. .PPKKPPRPG APAHLTNLS. ...PADSYNE GVK.LQPQEI Fugu1newFAK HRQA...... .PVLPELRTQ ALPLQGFVL. ...MTFSFSR VQ..LQPQEI Fugu2newFAK PVGKPEHVA. .PPKKPPRPS AQSNLACQN. ...TGDSYND GVK.LQPQEI HumanPyk2 EKEVGYLEFT GPPQKPPRLG AQSIQ..... .......... .......... ChimpPyk2 EKEVGYLEFT GPPQKPPRLG AQSIQ..... .......... .......... RatPyk2 EKEAGYTEFT GPPQKPPRLG AQSIQ..... .......... .......... MousePyk2 EKEAGYTEFT GPPQKPPRLG AQSIQ..... .......... .......... ChickenPyk2 VALPAPGALP VPHQKPPRLG AQSIQ..... .......... .......... FrogPyk2 EKELAYTEFT VPPQKPPRLG AQSLQ..... .......... ........PL ZebrafishPyk21 EPPLSYAELH HPPSKPPRIA VQPA...... .......... .......... ZebrafishPyk22 IISPVLFSSQ GPPEKPPRVS AQPA...... .......... .......... FuguPyk2 RCRAVLRLNA PLITVPKRGS MSVFYKVNLP VLLQDPMGSE ENV.....SP CionaFAK AVDVAPPPPS MPPAKPPRNP PIKDVQLFFV FIKHCSFQ.. ....PTFEEV SeaUrchinFAK TSAANQYYQD TDEVRRELPP EPSEQEMASH PPQQ...... ....PQPQPL DrosophilaFAK SSLKSNHSSA DHLSSLTSAE EQMGS..... .......... ....NARNLG AnophaeleFAK GGGMIKSKSS SMERCTTPQS GDEK...... .......... ....FAVKKV ApisFAK .......... .......... .......... .......... .......... CelegansFAK .......... .......... .......... ....SNGYLH HTP....... Hydra .......... .......... .......... .......... .......... Hydractinia EKKAKRNSTL RRSIPLWGFE RNDFANEKKP SPLAREVPPP TTPPGNESAI 1201 1250 HumanFAK SPP....... .......... .......... .......... .......... ChimpFAK SPP....... .......... .......... .......... .......... RatFAK SPP....... .......... .......... .......... .......... MouseFAK SPP....... .......... .......... .......... .......... ChickenFAK SPP....... .......... .......... .......... .......... FrogFAK SPP....... .......... .......... .......... .......... Zebrafish1aFAK NPP....... .......... .......... .......... .......... Zebrafish1bFAK SPP....... .......... .......... .......... .......... Fugu1newFAK SPT....... .......... .......... .......... .......... Fugu2newFAK SPP....... .......... .......... .......... .......... HumanPyk2 .......... .......... .......... .......... .......... ChimpPyk2 .......... .......... .......... .......... .......... RatPyk2 .......... .......... .......... .......... .......... MousePyk2 .......... .......... .......... .......... .......... ChickenPyk2 .PA....... .......... .......... .......... .......... FrogPyk2 QPT....... .......... .......... .......... .......... ZebrafishPyk21 .......... .......... .......... .......... .......... ZebrafishPyk22 .......... .......... .......... .......... .......... FuguPyk2 LPA....... .......... .......... .......... .......... CionaFAK KPT....... .......... .......... .......... .......... SeaUrchinFAK SAQ....... .......... .......... .......... .......... DrosophilaFAK SAV....... .......... .......... .......... .......... AnophaeleFAK EPT....... .......... .......... .......... .......... ApisFAK .......... .......... .......... .......... .......... CelegansFAK .......... .......... .......... .......... .......... Hydra .......... .......... .......... .......... .......... Hydractinia EPPSPPVITT DNVRDEDIQP IADVVDSAKP KLSRSNSASD TYAKPIKKKD 1251 1300 HumanFAK .......... .......... .......... .......... .PTANLDRSN ChimpFAK .......... .......... .......... .......... .PTANLDRSN RatFAK .......... .......... .......... .......... .PTANLDRSN MouseFAK .......... .......... .......... .......... .PTANLDRSN ChickenFAK .......... .......... .......... .......... .PTANLDRSN FrogFAK .......... .......... .......... .......... .PTANLDRTN Zebrafish1aFAK .......... .......... .......... .......... .PTANLDRSN Zebrafish1bFAK .......... .......... .......... .......... .PTANLDRSN Fugu1newFAK .......... .......... .......... .......... .PTANLDRSN Fugu2newFAK .......... .......... .......... .......... .PTANLDRSN HumanPyk2 .......... .......... .......... .......... .PTANLDRTD ChimpPyk2 .......... .......... .......... .......... .PTANLDRTD RatPyk2 .......... .......... .......... .......... .PTANLDRTD MousePyk2 .......... .......... .......... .......... .PTANLDRTD ChickenPyk2 .......... .......... .......... .......... .PTANLDRTD FrogPyk2 .......... .......... .......... .......... .PTANIDRTD ZebrafishPyk21 .......... .......... .......... .......... .PTAELDRSE ZebrafishPyk22 .......... .......... .......... .......... .PTAELDRTD FuguPyk2 .......... .......... .......... .......... .PTAELDRSD CionaFAK .......... .......... .......... .......... .PTMKLDREG SeaUrchinFAK .......... .......... .......... .......... .IAPTLDMDR DrosophilaFAK .......... .......... .......... .......... .PSRPPNRAD AnophaeleFAK .......... .......... .......... .......... .KTMPLDRTN ApisFAK .......... .......... .......... .......... .PTADLDRTN CelegansFAK .......... .......... .......... .......... .TSTRSLRFE Hydra .......... .......... .......... .......... .......... Hydractinia RLEETIPRCN TTGQIENKNS SPRLRTRSEQ KPLKKEKQEN KPTADV.... 1301 1350 HumanFAK DKVYENVTGL VKAVIEMS.S KIQPAPP.EE YVPMVKEVGL ALRTLLATVD ChimpFAK DKVYENVTGL VKAVIEMS.S KIQPAPP.EE YVPMVKEVGL ALRTLLATVD RatFAK DKVYENVTGL VKAVIEMS.S KIQPAPP.EE YVPMVKEVGL ALRTLLATVD MouseFAK DKVYENVTGL VKAVIEMS.S KIQPAPP.EE YVPMVKEVGL ALRTLLATVD ChickenFAK DKVYENVTGL VKAVIEMS.S KIQPAPP.EE YVPMVKEVGL ALRTLLATVD FrogFAK DKVYENVTGL VKAVIEMS.S RIQPAPP.EE YVPMVKGVGL ALRTLLATVD Zebrafish1aFAK DKVYENVTGL VKAVIEMS.S KIQPAPP.EE YVPMVKEVGL ALRTLLATVD Zebrafish1bFAK DKVYENVTGL VKAVIEMS.S RIQPALP.EE YVPMVKDVGL ALRTLLATVD Fugu1newFAK DKVYENVTGL VKAVIEMS.N RIQPAAP.EE YVPMVKEVGL ALRTLLATVD Fugu2newFAK DKVYENVTGL VKAVIEMS.S KIQPAPP.EE YVPMVKDVGL ALRTLLATVD HumanPyk2 DLVYLNVMEL VRAVLELK.N ELCQLPP.EG YVVVVKNVGL TLRKLIGSVD ChimpPyk2 DLVYLNVMEL VRAVLELK.N ELCQLPP.EG YVVVVKNVGL TLRKLIGSVD RatPyk2 DLVYHNVMTL VEAVLELK.N KLSQLPP.EE YVVVVKNVGL NLRKLIGSVD MousePyk2 DLVYHNVMTL VEAVLELK.N KLGQLPP.ED YVVVVKNVGL NLRKLIGSVD ChickenPyk2 DMVYSNVMDL VRAVLQLK.N EISILPP.EE YILVVKNVGL SLRKLISSVD FrogPyk2 DGVYQSVMEL VKAVLQLK.N DIYQLPP.EG YVAVVKNVGL TLRKLIGSVD ZebrafishPyk21 DTVYQCVMDV VKVVVQLK.N DINTLSS.TE YISPVKTVGL SLRDLINSVD ZebrafishPyk22 DLVYQNVMEM VKVVVQLK.N QVNTLPA.SE YVSVVKSVGM TLRSLIHSVD FuguPyk2 DNVYNSVMGL VKVVVQFK.N DVVDLKP.EQ YISLVQSVGV ALRDLIQSVD CionaFAK DAVYRDTMLV VKAVLQAN.Q EVMAAKP.DE IFVLVKNIGK ALKELCTSLD SeaUrchinFAK TDDYVFENTT Q..VVRSV.M EMVNLVHAVK SDQYVNLVRD IGLALKSLLS DrosophilaFAK DEVYCATTLV VKSIMALS.Q GVEKANT.EG YLELVKNVGV KLRNLLTSVD AnophaeleFAK DMVYNATTSV VKSIMALS.Q GVDRAQA.AE YLNLVRNVGF ELRALLGAVD ApisFAK DKVYDCTTSV VRAVMSLSQQ GVQQSKA.DQ YLELVRKVGI ELRALLSSVD CelegansFAK DKTSRGLRRS VDGVCDAVTK LQNSFNNLTH NDDFLHSVK. EVTSQLREML Hydra .......... .......... .......... .......... .......... Hydractinia ..VYQHTTSV VKSVIELN.T GVQHAQP.EE FVDLVKVVGL NXRDLLAEVD 1351 1400 HumanFAK ET...IPLLP AST..HREIE MAQKLLNS.D LGELINKMKL AQQYVMTSLQ ChimpFAK ET...IPLLP AST..HREIE MAQKLLNS.D LGELINKMKL AQQYVMTSLQ RatFAK ET...IPILP AST..HREIE MAQKLLNS.D LGELISKMKL AQQYVMTSLQ MouseFAK ET...IPALP AST..HREIE MAQKLLNS.D LGELISKMKL AQQYVMTSLQ ChickenFAK ES...LPVLP AST..HREIE MAQKLLNS.D LAELINKMKL AQQYVMTSLQ FrogFAK ET...IPVLP AST..HREIE MAQKLLNS.D LAELINKMKL AQQYVMTSLQ Zebrafish1aFAK ET...IPLLP AHT..HREIE MAQKLLNS.D LAELINKMKL AQQYVMTSLQ Zebrafish1bFAK ET...IPVLP AHT..HREIE MAQKLLNS.D LAELISKMRL AQQYMLTSLQ Fugu1newFAK ET...LPVLP AST..HREIE MAQKLLNS.D LAELISKMKL AQQYVMTSLQ Fugu2newFAK ET...LPQLP AST..HREIE MAQKLLNS.D LAELIGKMKL AQQYVMTSLQ HumanPyk2 DL...LPSLP SSS..RTEIE GTQKLLNK.D LAELINKMRL AQQNAVTSLS ChimpPyk2 DL...LPSLP SSS..RTEIE GTQKLLNK.D LAELINKMRL AQQNAVTSLS RatPyk2 DL...LPSLP ASS..RTEIE GTQKLLNK.D LAELINKMRL AQQNAVTSLS MousePyk2 DL...LPSLP ASS..RTEIE GTQKLLNK.D LAELINKMKL AQQNAVTSLS ChickenPyk2 EM...LPLLP ATS..RTEIE GTQKLLNK.D LAELINKMRL AQQNAVTSLS FrogPyk2 EI...LPSLP QNS..RTEIE GTQKLLSK.D LAELINKMRL AQQNAVTSLS ZebrafishPyk21 DI...LPTLH ISC..RTEIE GTQKLLNK.D LAELISKMRL AQQNAITSLK ZebrafishPyk22 DI...LPSLH ESI..RTEIE GTQKLLNK.D LAELISKMRL AQQNSVTSLK FuguPyk2 EI...LPMLH ESV..RTEIE GTEKLLNN.D MAELISKMRL AQQNAITSLK CionaFAK QE...TPHLP PEY..HREID MAQKTTNK.D LSQVINQMKL VKRFYSTTQV SeaUrchinFAK SVDTLYSDLP GET..HRGIQ LAHKVLSS.D MGELINAMKL AQKYATTTLD DrosophilaFAK KISIIFPAQA L.....KEVQ MAHQVLSK.D MHELVSAMRL AQQYSDTTLD AnophaeleFAK QLSANFPPQR .....YKEVE MAHKVLSK.D MYELVTAMRL AQQYSETTLD ApisFAK ALMDILPISA .....HREVE MAHKVLSK.D MAELVTAMKQ AQKYSATTLD CelegansFAK IVASGMRDRV TTTTQRTDVD MT.KTLIAND MKQMSRVMGK LQVNGHQATY Hydra .......... .......... .......... .......... .......... Hydractinia KE...IQLVP SIS..QKEIE MAHKVLSS.D MAELVNXMKL AQKYADPTLD 1401 1450 HumanFAK QEYKKQMLTA AHALAVDAKN LLDVIDQARL KMLGQTRPH. .......... ChimpFAK QEYKKQMLTA AHALAVDAKN LLDVIDQARL KMLGQTRPH. .......... RatFAK QEYKKQMLTA AHALAVDAKN LLDVIDQARL KMLGQTRPH. .......... MouseFAK QEYKKQMLTA AHALAVDAKN LLDVIDQARL KMLGQTRPH. .......... ChickenFAK QEYKKQMLTA AHALAVDAKN LLDVIDQARL KMISQSRPH. .......... FrogFAK QEYKKQMLTA AHALAVDAKN LLDVIDQARL KIISHSRPH. .......... Zebrafish1aFAK QDYKKQMLTA AHALAVDAKN LLDVIDQARL KLLGQARPH. .......... Zebrafish1bFAK QDYKKQMLTA AHALAVDAKN LLDVIYQARL KM.SH.RTH. .......... Fugu1newFAK KDYKKHMLMA AHALAVDAKN LLDVIDQSRL KMI....... .......... Fugu2newFAK QDYKKQMLTA AHALAVDAKN LLDVIDQSRL KMMAHSRPH. .......... HumanPyk2 EECKRQMLTA SHTLAVDAKN LLDAVDQAKV LANLAHPPAE .......... ChimpPyk2 EECKRQMLTA SHTLAVDAKN LLDAVDQAKV LANLAHPPAE .......... RatPyk2 EDCKRQMLTA SHTLAVDAKN LLDAVDQAKV VANLAHPPAE .......... MousePyk2 EDCKRQMLTA SHTLAVDAKN LLDAVDQAKV VANLAHPPAE .......... ChickenPyk2 EECKKQMLTA SHTLAVDAKN LLDAVDQAKV QANLVKLCLE .......... FrogPyk2 EECKKQMLTA SHTMAVDAKN LLDAVDQARV RANLAKVAAE .......... ZebrafishPyk21 EECKKQMLTA AHTLAMDAKN LLDAVDQARV RSNLAKPKPD DPDSPAD... ZebrafishPyk22 DECKKQMLAA AHTLAMDSKN LLDAVDQARV RANTARP... .......... FuguPyk2 EECKKQMLSA AHTLAMDSKN LLDAVDQARV RANIAKP... .......... CionaFAK TDYKKCMMAA TQVLAMDAKN LLDVVDKSRL QALAGAS... .......... SeaUrchinFAK QDYKRGMIQA GHVLTVDAKN LLDAVDTARL QAQDLVMGGA RQPEELS... DrosophilaFAK CEYRKSMLSA AHVLAMDAKN LFDVVDSIRQ RYQHLFPPSA TKETSCSSSF AnophaeleFAK AEYRKSMLSA AHVLAMDAKN LLDVVDSIRV RYPNLFP... .......... ApisFAK AEYRKGMLSA AHILAMDAKN LLDVIDSIRI RYPY...... .......... CelegansFAK NTLRRDVVRI CGELAVNCTT LQLQLTQPPL ENEFSSLLSN C......... Hydra .......... .......... .......... .......... .......... Hydractinia QQYRRNMLAA AHALAIDAKN LYDTFSKLKN KVLEET.... .......... 1451 1500 HumanFAK .......... .......... .......... .......... .......... ChimpFAK .......... .......... .......... .......... .......... RatFAK .......... .......... .......... .......... .......... MouseFAK .......... .......... .......... .......... .......... ChickenFAK .......... .......... .......... .......... .......... FrogFAK .......... .......... .......... .......... .......... Zebrafish1aFAK .......... .......... .......... .......... .......... Zebrafish1bFAK .......... .......... .......... .......... .......... Fugu1newFAK .......... .......... .......... .......... .......... Fugu2newFAK .......... .......... .......... .......... .......... HumanPyk2 .......... .......... .......... .......... .......... ChimpPyk2 .......... .......... .......... .......... .......... RatPyk2 .......... .......... .......... .......... .......... MousePyk2 .......... .......... .......... .......... .......... ChickenPyk2 .......... .......... .......... .......... .......... FrogPyk2 .......... .......... .......... .......... .......... ZebrafishPyk21 .......... .......... .......... .......... .......... ZebrafishPyk22 .......... .......... .......... .......... .......... FuguPyk2 .......... .......... .......... .......... .......... CionaFAK .......... .......... .......... .......... .......... SeaUrchinFAK .......... .......... .......... .......... .......... DrosophilaFAK ESTSGSIVTE PVNDLGGYIK TSTSGDLLQN TGIYDNDLHH SFNSQLQLQN AnophaeleFAK .......... .......... .......... .......... .......... ApisFAK .......... .......... .......... .......... .......... CelegansFAK .......... .......... .......... .......... .......... Hydra .......... .......... .......... .......... .......... Hydractinia .......... .......... .......... .......... .......... 1501 1550 HumanFAK .......... .......... .......... .......... .......... ChimpFAK .......... .......... .......... .......... .......... RatFAK .......... .......... .......... .......... .......... MouseFAK .......... .......... .......... .......... .......... ChickenFAK .......... .......... .......... .......... .......... FrogFAK .......... .......... .......... .......... .......... Zebrafish1aFAK .......... .......... .......... .......... .......... Zebrafish1bFAK .......... .......... .......... .......... .......... Fugu1newFAK .......... .......... .......... .......... .......... Fugu2newFAK .......... .......... .......... .......... .......... HumanPyk2 .......... .......... .......... .......... .......... ChimpPyk2 .......... .......... .......... .......... .......... RatPyk2 .......... .......... .......... .......... .......... MousePyk2 .......... .......... .......... .......... .......... ChickenPyk2 .......... .......... .......... .......... .......... FrogPyk2 .......... .......... .......... .......... .......... ZebrafishPyk21 .......... .......... .......... .......... .......... ZebrafishPyk22 .......... .......... .......... .......... .......... FuguPyk2 .......... .......... .......... .......... .......... CionaFAK .......... .......... .......... .......... .......... SeaUrchinFAK .......... .......... .......... .......... .......... DrosophilaFAK PKASIDLSGG GSLQRGMSLG LDTTRSTNEP LRIVEETLGS PGEHMYCNTS AnophaeleFAK .......... .......... .......... .......... .......... ApisFAK .......... .......... .......... .......... .......... CelegansFAK .......... .......... .......... .......... .......... Hydra .......... .......... .......... .......... .......... Hydractinia .......... .......... .......... .......... .......... 1551 1556 HumanFAK ...... ChimpFAK ...... RatFAK ...... MouseFAK ...... ChickenFAK ...... FrogFAK ...... Zebrafish1aFAK ...... Zebrafish1bFAK ...... Fugu1newFAK ...... Fugu2newFAK ...... HumanPyk2 ...... ChimpPyk2 ...... RatPyk2 ...... MousePyk2 ...... ChickenPyk2 ...... FrogPyk2 ...... ZebrafishPyk21 ...... ZebrafishPyk22 ...... FuguPyk2 ...... CionaFAK ...... SeaUrchinFAK ...... DrosophilaFAK ALHGHA AnophaeleFAK ...... ApisFAK ...... CelegansFAK ...... Hydra ...... Hydractinia ......