Benzo[a]pyrene diol epoxide adducts in DNA are potent suppressors of a normal topoisomerase I cleavage site and powerful inducers of other topoisomerase I cleavages -- Data Supplement - Supplemental Fig. 6 -- Proceedings of the National Academy of Sciences

Supplementary material for Pommier et al., Proc. Natl. Acad. Sci. USA, 10.1073/pnas.040397497

Fig. 6.
CD spectra [in 0.02 M phosphate buffer (pH 7), with ionic strength adjusted to 0.1 M with NaCl] of the synthesized oligonucleotide 22-mers containing cis and trans opened BaP DE-2 adducts at the indicated dG* residue. Spectra are normalized to 1.0 absorbance unit at 260 nm. Absolute configurations of the oligonucleotides containing cis opened adducts were assigned on the basis of the long wavelength (300-370 nm) pyrene bands (positive for 10R and negative for 10S adducted oligonucleotides). For the oligonucleotides containing trans opened adducts, in which these bands were weak, configurational assignments were confirmed by enzymatic hydrolysis to the known nucleoside adducts (see text).