Guanylyl Cyclase Protein and cGMP Product Independently Control Front and Back of Chemotaxing Dictyostelium Cells -- Veltman and Van Haastert 17 (9): 3921 Data Supplement - Supplemental Material -- Molecular Biology of the Cell

Guanylyl Cyclase Protein and cGMP Product Independently Control Front and Back of Chemotaxing Dictyostelium Cells
Mol. Biol. Cell Veltman and Van Haastert 17: 3921

Supplemental Material

This article contains the following supporting material:

Movie 1 - Localization of sGCΔcat-GFP during chemotaxis
Cells expressing the catalytically inactive sGCΔcat-GFP were starved for 5 hours and allowed to migrate towards a micropipette filled with 10^-4 M cAMP. The location of the tip of the pipette is marked by the asterisk. Images were collected at 10 second intervals and the movie shows 6 frames per second, resulting in a 60-fold time acceleration.