Supporting Figures

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Fig. 6. Room temperature FTIR and photosynthesis spectra of ¹²CO-bound L92F and L92V full-length HemAT mutants, pH 8.5. (a) FTIR spectra of the CO-bound L92F and L92V full-length HemAT mutants. (b) Light (416 nm)-minus-dark FTIR difference spectra of the CO-bound L92F and L92V full-length HemAT mutants.

Fig. 7. Room temperature FTIR spectra of sensor domain HemAT. FTIR spectra of the ¹²CO-sensor domain (spectrum A) and ¹³CO-sensor domain (spectrum B) HemAT at pD 8.5. Spectrum C is the ¹³CO-bound Y133F mutant. pD = pH + 0.4.

Fig. 8. Room temperature photolysis FTIR spectra of sensor domain HemAT. Light (416 nm)-minus-dark FTIR difference spectra of ¹²CO-bound (spectrum A) and ¹³CO-bound (spectrum B) sensor domain HemAT at pD 8.5. Spectrum C is the light-minus-dark FTIR spectrum of the ¹³CO-bound Y133F mutant. pD = pH + 0.4.

Fig. 9. Plot of C-O optimized distances of free carbon monoxide in models A, A1-A8, B, C, and D versus , where n is the vibrational frequency of CO stretch. The plot shows a linear relation, demonstrating consistency with Badger's rule.

Fig. 10. Light (416 nm)-minus-dark FTIR difference spectra of the CO-bound Y133F full-length HemAT.