Activin receptor-like kinase 1 modulates transforming growth factor-beta 1 signaling in the regulation of angiogenesis -- Data Supplement - Supplemental Fig. 6 -- Proceedings of the National Academy of Sciences

Supplementary material for Oh et al. (2000) Proc. Natl. Acad. Sci. USA 97 (6), 2626-2631.

Fig. 6.
Representative semi-log view of real-time amplification plots of b-actin (A), a 1 type IV collagen (Col4a1) (B), vascular endothelial growth factor (VEGF) (C), and tissue-type plasminogen activator (tPA) (D) during 40 PCR cycles. For each gene, several dilutions of wild-type (WT) cDNAs (10´, 1´, 0.1´, and 0.01´) were used to measure the relative amount of transcripts in homozygous (-/-; red), heterozygous (+/-; green), and WT (yellow) embryonic day (E)9.5 embryos. After initial normalization with b-actin primer, cDNA amount from each genotype that is equivalent to 1´ WT cDNA was used for the PCR amplification. (A) PCR products amplified by b-actin primers using cDNA from WT, +/-, and -/- E9.5 embryos were well aligned to 1´ cDNA from WT, verifying the normalization of the initial amount of total cDNA for the amplification. (B) The amplification plot indicates that Col4a1 transcripts were unaltered in activin receptor-like kinase (ALK)1^+/- embryos. (C and D) The amplification plot of VEGF (C) and tPA (D) in -/- (red) was shifted to left (10´ WT), indicating elevated levels of VEGF and tPA transcripts in ALK1^-/-, compared to wild-type and ALK1^+/- embryos.