Dreanno et al. 10.1073/pnas.0602763103.

Supporting Figures

Files in this Data Supplement:

Supporting Figure 5
Supporting Figure 6




Supporting Figure 5

Fig. 5. Molecular analysis of the SIPC. Amino acid sequence alignment of the SIPC and the a2-macroglobulin (A2M) family. Identical and functionally similar amino acids are denoted by black and gray shading, respectively. The proteolytic fragments of the SIPC are indicated by a thick line for the 88- and 98-kDa subunits and by a dashed line for the 76-kDa subunit. GenBank GI and accession nos.: Ornithodoros moubata, gi22901939; Limulus sp., gi7521905; Balanus amphitrite, AY423545.





Supporting Figure 6

Fig. 6. The relationship of the Balanus amphitrite SIPC to the thioester-containing protein family. The unrooted phylogenetic tree was built by the neighbor-joining method on amino acid Poisson distances. The percentage confidence scores of a bootstrap test of 1,000 replicates are indicated for the major nodes. GenBank database GI numbers of the sequences used in the analysis are listed in parentheses: Anopheles gambiae TEPI (9837560); Branchiostoma belcheri C3 (13928544); Caenorhabditis elegans hypothetical protein ZK337.1b (7511093); Cyprinus carpio C4-1 (9453863), C3-Q2 (7512125), C5-2 (24785187), A2M-1 (6009727); Cavia porcellus murinoglobulin (2144133); Ciona intestinalis C3-1 (18074013), C3-2 (18074015), A2M (19032251); Drosophila melanogaster TEP1 (7573501), TEP2 (7573504), TEP3 (7573506), TEP4 (7573508); Eptatretus burgeri A2M (45259534), C3 (62774); Gallus gallus C3 (2118406), C4 (7512215); Halocynthia roretzi C3 (4579689); Homo sapiens A2M (4557225), pregnancy-zone protein (4506355), CD109 (19424130), C3 (116594), C4A (443671), C4B (1314244), C5 (116607); Lethenteron japonicum A2M (11273478), C3 (222888); Limulus sp. A2M (7521905); Mus musculus murinoglobulin (6678966), A2M (2492496), C3 (28175786), C4 (116603), C5 (116608); Oncorhynchus mykiss C3 (2118417); Ornithodoros moubata A2M (22901939); Rattus norvegicus a-1-inhibitor III (112893); Strongylocentrotus purpuratus C3 (2832239); Sus scrofa C3 (11869931); Swiftia exserta C3-like protein (27357203); Triakis scyllium C4 (34097968); Xenopus laevis endodermin (1929449), C3 (758425), C4 (1183857).