Litvinov et al. 10.1073/pnas.0603057103. |
Fig. 6. AR engagement by synthetic androgen ligand stimulates growth of LNCaP, CWR22Rv1, and LAPC-4 prostate cancer cell lines but has no effect on the growth of a PrSC cell line. Such effect can be reversed by addition of AR antagonist, Casodex. 100% (i.e., maximal) growth for these androgen-sensitive cells was defined as the growth in the presence of 1 nM R1881.
Fig. 7. Dual-parameter FACS analysis of AR expression in LAPC-4 cells. Exponentially growing LAPC-4 cells in the presence of 1 nM R1881 were analyzed by FACS as described in Fig. 2. LAPC-4 cells exhibit the same pattern of AR fluctuation as do LNCaP and CWR22Rv1 cells (A). Forward-side scatter analysis (B) of LAPC-4 cells documented that population A cells, which express undetectable AR levels, are the smallest and the least complex cells in culture, which is consistent with them being postmitotic, early G1 cells. Population C cells, which express the highest levels of AR protein and exhibit 4 N DNA fluorescence, are the largest and most complex cells in culture, which is consistent with them being late G2 cells. Population B cells, which express high AR levels and correspond to G1 phase state on the basis of DNA fluorescence, fall in between the A and C populations on the basis of size and complexity.