Supplementary material for Singh et al., Proc. Natl. Acad. Sci. USA, 10.1073/pnas.050583297

Table 2.

 Reverse transcription (RT)–PCR analysis of neuronal nitric oxide synthase and androgen receptor

Gene

Primers

Conditions

nNOS

Sense, 5'-GAA TAC CAG CCT GAT CCA TGG GAA-3'

Antisense, 5'-TCC TCC AGG AGG GTG TCC ACC GCA TC-3'

Ref. 1

35 cycles

94

°C for 1 min (denaturation)

60

°C for 1 min (annealing)

72

°C for 1.5 min (extension)

AR

Sense, 5'-GAG CGT GCG CGA AGC GAT CCA GAA-3'

Antisense, 5'-TGC TGC CTT CGG ATA TTA CCT CCT GCT-3'

Ref. 2

Same as nNOS

GAPDH

Sense, 5'-GTG AAG GTC GGT GTC AAC GGA TTT-3'

Antisense, 5'-CAC AGT CTT CTG AGT GGC AGT GAT-3'

Ref. 3

30 cycles, 500 ng total cellular RNA

94

°C for 30 sec (denaturation)

55

°C for 30 sec (annealing)

72

°C for 1 min (extension)

GAPDH, glyceraldehyde-3-phosphate dehydrogenase.

 

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3. Tso, J. Y., Sun, X. H., Kao, T. H., Reece, K. S. & Wu, R. (1985) Nucleic Acids Res. 13, 2485–2502.