Supplemental Material

This article contains the following supporting material:

• Figure 1
• Figure 2
• Supplemental Material - Tables 1-3

• Movie 1 - Wild type yeast cells expressing Abp1p-3x GFP were grown for 2 days at 30°C in SD casa medium. After a brief centrifugation, the cell pellet was resuspended in fresh medium and cells were immediately imaged. Images are 2D maximal projection of 3D image stacks time laps series. Time after addition of fresh medium is indicated.

• Movie 2 - Wild type yeast cells expressing Abp1p-3x GFP were grown for 2 days at 30°C in SD casa medium. After a brief centrifugation, the cell pellet was resuspended in fresh medium and cells were immediately imaged. Images are 2D maximal projection of 3D image stacks time laps series. Time after addition of fresh medium is indicated. The histogram on the bottom shows the fluorescence intensity of the Abp1p-3xGFP signal in pseudo color at the corresponding time point.

• Movie 3 - Wild type yeast cells expressing Sac6p-3x GFP were grown for 2 days at 30°C in SD casa medium. After a brief centrifugation, the cell pellet was resuspended in fresh medium and cells were immediately imaged. Images are 2D maximal projection of 3D image stacks time laps series. Time after addition of fresh medium is indicated. The histogram on the bottom shows the fluorescence intensity of the Sac6p-3xGFP signal in pseudo color at the corresponding time point.

• Movie 4 - Wild type yeast cells expressing Abp1p-3x GFP were grown for 3 days at 30°C in SD casa medium. The cell culture was concentrated and Lat-A was added to the final concentration of 200 μM. This concentrated culture was incubated 30 min at 30°C. After this incubation, 20 μl of the cell suspension was mixed to 20 μl of 2X fresh SD casa medium containing 200 μM Lat-A and immediately imaged. Images are 2D maximal projection of 3D image stacks time laps series. Time in minutes after addition of Lat-A containing water or fresh medium is indicated.