Supporting Figures

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Fig. 9. Fluorescence spectra of Sso7d (16 mM) in the presence of either ODN2 or ODN4 (32 mM). Fluorescence spectra were recorded by using an excitation wevelength of 295 nm. Sample solutions contain 50 mM sodium cacodylate buffer (pH 7.0).

Fig. 10. HPLC profile of photoirradiated ODN3 with or without acetophenon. (A) Before photoirradiation. (B) Photoirradiation with transilluminator light at 302 nm in the absence of acetophenone. (C) Photoirradiation with monochrometer light at 330 nm in the absence of acetophenone. (D) Photoirradiation of ODN3 with monochrometer light at 330 nm in the presence of acetophenone (5 mM). Reaction conditions are as follows. The reaction mixture (total volume, 70 ml) contained ODN3 (80 mM strand concentration) in 50 mM sodium cacodylate buffer (pH 7.0). Irradiation was performed with a transilluminator light at 302 nm for 10 min (B) or with a monochrometer light at 330 nm for 60 min at 0°C (C and D). The reaction mixtures were analyzed by HPLC on a Chemcobond 5-ODS-H column (4.6 ´ 150 mm) with detection at 254 nm; elution was performed with 0.05 M ammonium formate (pH 6.5) containing 3-10% acetonitrile over a linear gradient for 30 min at a flow rate of 1.0 ml/min, at 40°C.