Supporting Information

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Fig. 5. Concentration dependence of vimentin assembly. (A) Scattering from the K139C mutant in 20 mM NaCl and 5 mM Tris·HCl (pH 8.4) at 2.5, 6.5, and 18 mg/ml protein (blue circles, red squares, and green triangles, respectively). (B) Corresponding p_cr(r) functions.

Fig. 6. Refinement of tetramer and octamer models. (A) Goodness-of-fit between the calculated scattering from the tetramer model and the experimental data as a function of the distance d_tetra between the dimers' axes. (B) 2D plot showing the goodness-of-fit between the calculated and experimental scattering curves as a function of the separation between the adjacent dimers d_octa and the angle a (see Fig. 3D for definitions).

Fig. 7. Example of SAXS data interpretation for polydisperse samples. Shown are experimental scattering curves for solutions of vimentin K139C mutant in 20 mM NaCl and 5 mM Tris·HCl (pH 8.4) at 2.5, 6.5, and 18 mg/ml (blue, green. and red dots, respectively) together with calculated scattering for the corresponding mixtures of oligomers (see Table 1). The two latter curves were vertically displaced for visibility.

Movie 1. Final vimentin tetramer model.

Movie 2. Final vimentin octamer model.

Movie 3. Final vimentin unit-length filament model.