Proinsulin is encoded by an RNA splice variant in human blood myeloid cells -- Narendran et al. 103 (44): 16430 Data Supplement - HTML Page - index.htslp -- Proceedings of the National Academy of Sciences

Narendran et al. 10.1073/pnas.0607380103.

Supporting Tables

Table 1. Oligonucleotide primers and hybridization probes

Description	Sequence (5¢-3¢ )
Nested PCR
5¢ external proinsulin primer	GAAGAGGCCATCAAGCAGATCACT
5¢ internal proinsulin primer	ACCTGACCCAGCCGCAGCC
3¢ internal proinsulin primer	AGCTGGTAGAGGGAGCAGATG
5¢ external GAD65 primer	TGCTCCAAAGTGGATGTCAACTA
3¢ external GAD65 primer	ATGTTAGTATTTGCTGTTGATGTCA
5¢ internal GAD65 primer	CTTTGGCGTTTCTGCAAGAT
3¢ internal GAD65 primer	AGTTGTTTGGCAATGCATCA
5¢ external 21-OH primer	ACCTGTCCTTGGGAGACTAC
3¢ external 21-OH primer	TAGGCAGGCATTAAGTTGTCG
5¢ internal 21-OH primer	AGCCCACAAGAAGCTCACC
3¢ internal 21-OH primer	TGATCTTGTCTCCGAAGGTG
Real-Time (Lightcycler) PCR
5¢ native proinsulin primer	AGAGGCCATCAAGCACATCACT
3¢ native proinsulin primer	GGGTGTGTAGAAGAAGCCTCGT
5¢ splice variant proinsulin primer	TGCGTCAGATCACTGTCCTTC
FL-proinsulin hybridization probe	AACCAACACCTGTGCGGCTCAC
LC Red 640-proinsulin hybridization probe	CCTGGTGGAAGCTCTCTACCTAGTGTGC
5¢ 21-OH primer	ACACGGCCCAGCAGCA
3¢ 21-OH primer	GGAAGCGATCAGGCCAGAA
FL-21-OH hybridization probe	GGTTCGGAATGATGACTGTGCCC
LC Red 640-21-OH hybridization probe	CAGGGATGTCGTAGCCGGAGAT
5¢ PBGD primer	GCTGCAACGGCGGAA
3¢ PBGD primer	CCTGTGGTGGACATAGCAATGATT
FL-PBGD hybridization probe	TCGCATACAGACGGACAGTGTGGTG
LC Red 640-PBGD hybridization probe	CAACATTGAAAGCCTCGTACCCTGG

Table 2. si RNA sequences

siRNA no.	DNA sequence
1	Sense gcucucuaccuagugugcgTT
	Antisense cgcacacuagguagagagcTT
2	Sense ggcuucuucuacacacccaTT
	Antisense uggguguguagaagaagccTC
3	Sense gccuuugugaaccaacaccTT
	Antisense gguguugguucacaaaggcTG
1 scrambled (negative control)	Sense cgucgucguacucauggucTT
	Antisense gaccaugaguacgacgacgTT