Supporting information for Correia et al. (2003) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.1131007100

 

Supporting Methods

Primer Sequences.

The coding portions and flanking DNA of the seven exons of fng were amplified by PCR using Herculase polymerase (Stratagene) and sequenced using the primers below, where "a" designates a forward primer, "b" designates a reverse primer, and the number(s) designate the exon(s) amplified and sequenced by the primer.

Fng 1a: TCGCTGGAAGAAGAGAAGAGTAGA;

Fng 1b: GTCGCCGGCGTTGATTTATGAA;

Fng 2a: TAGGAATAGGGTGACTCGAAATACTA;

Fng 2b: GCCCACCCGCCGACCTG;

Fng 34a: TGTTTGAATAATTATGCCGTCGTC;

Fng 34b: TTCAGCCTCCATATCGTTTCTCTT;

Fng 3b: AGGTCGGTCGGTTGCTGTTTG;

Fng 4b: GCGGCGGCGGCTGTCAAACGATAC;

Fng 5a: GGGCACACCCAAAGCAATCCT;

Fng 5b: TTGGGCCGGGCCAGAAGAT;

Fng 67a: TTCTCTGGGCCATTGCCAAAAAC;

Fng 67b: CTAAAAGGGATGTTGACATTTCTTGA;

Fng 6: AAGCGCTTGGGATCGGTTTTC;

Fng 7: CTACGCACTAAGCCGATTGGAAGA;

Fng 67a1: TCTGGGCCATTGCCAAAAA.