Supporting information for Giardini et al. (2003) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.1031670100
Movie 2.
Movement of an unusually large vesicle in Xenopus extract. Phase contrast (Left) and rhodamine-actin (Right) fluorescence images of a giant vesicle being squeezed by the comet tail. The approximate dimensions of this vesicle are 30 (length) × 15 µm (breadth). The overall shape of this vesicle is tear-drop, with a long actin-associated tubule extending from the back. The rear portion of this vesicle is highly contoured and compressed. The front of the vesicle appears round and smooth. Note that the shape of this vesicle is not as symmetrical as smaller vesicles (see Fig. 1B) (sped up 750 times real time). Frame dimensions are 55 × 55 µm.