Supporting information for Brady (2003) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.1137809100

 

Table 2. Primers used for both PCR amplification and DNA sequencing of gene fragments

 

Locus/

Primer

Direction

Sequence 5 ' à 3 '

Position

Source/ References

COI*

    

CI13

Forward

ATAATTTTTTTTATAGTTATACC

1986-2005†

1

CI14

Reverse

ATTTCTTTTTTTCCTCTTTC

2596-2571†

1

Jerry

Forward

CAACATTTATTTTGATTTTTTGG

2484-2506†

2

Ben3R

Reverse

GCWACWACRTAATAKGTATCATG

2914-2892†

3

18S

    

rc18A

Forward

TGGTTGATCCTGCCAGTAG

5-23‡

4

18N

Reverse

CACTCTAATTTKTTCAAAG

847-829‡

4

rc18H

Forward

GCTGAAACTTAAAGGAATTGACGGAAGGGCAC

1215-1246‡,§

4

18L

Reverse

CACCTACGGAAACCTTGTTACGACTT

1975-1950‡,§

 

28S

    

28SA

Forward

CCCCCTGAATTTAAGCATAT

3318-3337‡

B. Sullender, Rice University, Houston

28SC

Reverse

CGGTTTCACGTACTCTTGAA

3692-3673‡

B. Sullender

Bel28S

Forward

AGAGAGAGTTCAAGAGTACGTG

3665-3686‡

5

revBel28S

Reverse

TTGGTCCGTGTTTCAAGACGGG

4068-4047‡

5

wingless

    

wg1

Forward

GARTGYAARTGYCAYGGYATGTC

TGG

n/a

6

wg2

Reverse

ACTICGCRCACCARTGGAATGTRCA

n/a

6

*COI sequences were not obtained for the following taxa in the divergence dating analysis: Amblyopone australis, Liometopum occidentale, Myrmecia fulvipes, Dorylus helvolus, Aenictus rotundatus, Neivamyrmex nigrescens, N. carettei, N. romandii, N. augustinodis, and N. pilosus.

†

Position denotes coordinates in the Apis mellifera mitochondrion (7).

‡

Position denotes coordinates in Drosophila melanogaster using the numbering of Tautz et al. (8) as corrected by Linares et al. (9).

§

Not used in divergence dating analysis.

Amplifies total of 436 nucleotide positions.

 

1. Hasegawa, E., Tinaut, A. & Ruano, F. (2002) Ann.. Zool. Fenn. 39, 267-271.

2. Simon, C., Frati, F., Beckenbach, A., Crespi, B., Liu, H. & Flook, P. (1994) Ann.. Entomol. Soc. Am. 87, 651-701.

3. Brady, S. G., Gadau, J. & Ward, P. S. (2000) in Hymenoptera. Evolution, Biodiversity, and Biological Control, eds. Austin, A. D. & Dowton, M. (CSIRO Publishing, Collingwood, Victoria, Australia), pp. 131-139.

4. Wiegmann, B. M., Mitter, C., Regier, J. C., Friedlander, T. P., Wagner, D. M. & Nielsen, E. S. (2000) Mol. Phylogenet. Evol. 15, 242-259.

5. Belshaw, R. & Quicke, D. L. J. (1997) Mol. Phylogenet. Evol. 7, 281-293.

6. Brower, A. V. Z. & DeSalle, R. (1998) Insect Mol. Biol. 7, 73-82.

7. Crozier, R. H. & Crozier, Y. C. (1993) Genetics 133, 97-117.

8. Tautz, D., Hancock, J. M., Webb, D. A., Tautz, C. & Dover, G. A. (1988) Mol. Biol. Evol. 5, 366-376.

9. Linares, A. R., Hancock, J. M. & Dover, G. A. (1991) J. Mol. Biol. 219, 381-390.