Mice lacking methyl-CpG binding protein 1 have deficits in adult neurogenesis and hippocampal function -- Data Supplement - Supporting Information -- Proceedings of the National Academy of Sciences

Supporting information for Zhao et al. (2003) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.1131928100

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Fig. 6.
Generation of MBD1^–/–mice. (a) Schematic structure of mouse MBD1 protein. Shaded areas indicate functional domains. MBD, Methyl-CpG binding domain; CXXC, zinc finger motifs; TRD, transrepression domain. (b) Gene targeting strategy. Exons 2–10 were deleted in the mutant allele and the LacZ gene was fused in-frame with the MBD1 coding sequence. (c) Genomic Southern blot to detect homologous recombination in embryonic stem cells. Genomic DNA was digested with XbaI and hybridized with probe as shown in b. (d) Mice were genotyped by using PCR. +/+, WT; +/–, MBD1^+/–; and –/–, MBD1^–/–.