Supporting Figure 3

Fig. 3.

 A molecular phylogenetic tree of glutathione-S transferase genes. Best 200 entries obtained by a blastx (1) search using the sequence of putative transcript P006275 and two EST entries obtained by tblastn search using the deduced amino acid sequence coded by P006275 were aligned with P006275 using mafft-3.81 (2). The alignment was handled with macclade 4.05 on Mac OS X (3). Well aligned regions were selected for phylogenetic analysis and entries having large deletion in that regions were excluded. Unaligned regions were excluded, and redundant entries having the same amino acid sequence in the selected region were unified. The final alignment contained 105 amino acid sites of 157 sequences. A tree was obtained by njdist with the Neighbor Joining method (4) based on maximum likelihood distance under JTT model (5) calculated by protml. A local rearrangement search was further performed with protml to obtain the tree shown in the figure. njdist and protml were in MOLPHY 2.3 package (6). Sequences are shown with the name of organism and GI number in parenthesis. Local bootstrap probabilities estimated by the RELL (resampling of estimated log-likelihood) method (7, 8) are shown on each branch. Horizontal lengths are proportional to the estimated evolutionary distance. Vertical lengths are arbitrary. P006275 is on the uppermost branch.

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