A macrophage receptor for apolipoprotein B48: Cloning, expression, and atherosclerosis -- Data Supplement - Supplemental Data -- Proceedings of the National Academy of Sciences

Supplementary material for Brown et al., Proc. Natl. Acad. Sci. USA, 10.1073/pnas.120184097

Supplemental Data Fig. 8.
cDNA nucleotide sequence and derived amino acid sequence of the THP-1 monocyte apoB48R. Nucleotides are numbered at the right (top) with every 10 bp indicated by a V. The amino acid residues are also numbered at the right with 20 residues per line. Ten bp precede the ATG start codon; a Kozak start sequence is at bp 5-14. The entire cDNA sequence is 3,744 bp, whereas the derived protein length is 1,088 residues, with the TGA stop codon at bp 3,275. The 10-residue unambiguous amino acid sequence (amino acids 910-919) determined from microsequence analysis of the tryptic hydrolysis of purified apoB48R [Bradley, W. A., Brown, M. L., Ramprasad, M. P., Li, R., Song, R. & Gianturco, S. H. (1999) J. Lipid Res. 40, 744-752] and used to design the degenerate oligonucleotide primers is indicated by a double underline, as are two other tryptic peptides likewise identified. The single-underlined 23 amino acids (amino acids 751-773) make up the putative transmembrane domain. Cysteine residues are bold and underlined (C); potential coiled-coil interactive domains are bold and italicized (amino acids 155-189, amino acids 473-486, and amino acids 520-533). There are two potential polyadenylation signals beginning at bp 3,700 and bp 3,708. Both strands of the cDNA were sequenced; additional sequencing runs were carried out in some cases in regions with high CG content.