NMR structure of the active conformation of the Varkud satellite ribozyme cleavage site -- Data Supplement - HTML Page - 2440Fig7Legend.htslp -- Proceedings of the National Academy of Sciences

Supporting Figure 7

Fig. 7.
(a) Overlay of the ¹H-¹³C CT-HSQC spectra (1) of SL1' collected on the control sample (10 mM d11-Tris, pH 7.0, 50 mM NaCl, 0.05 mM NaN₃; black peaks) and on a sample supplemented with 10 mM MgCl₂ (red peaks). These spectra were recorded with aliasing in the ¹³C dimension; the C1', C2', C3', C4, C5', and C5 chemical shifts are indicated on the right, and the C2, C6, and C8 chemical shifts are indicated on the left. (b and c) Representative data showing the effect of MgCl₂ on individual chemical shifts and the nonlinear fit used to determine apparent K_ds for Mg²⁺ (see Methods). (b) Apparent K values for Mg²⁺ were determined for A14 H2 (K_d = 3.60; diamond) and A19 H2 (K_d = 0.99; triangle). (c) Apparent K_ds for Mg²⁺ were determined for A7 C3' (K_d = 3.05; circle) and A11 C3' (K_d = 3.73; diamond).

1. Santoro, J. & King, G. C. (1992) J. Magn. Reson. 97, 202–207.