Table 1. PCR primers used for mapping and analysis of the mouse Picalm gene

Gene/ marker	Type*	Forward primer	Forward primer sequence	Reverse primer	Reverse primer sequence	Product Size, bp
M-09035	Genomic	138	GCAGAAGGGAAGACAGTTGC	139	AGCCGTGAAGAGAGGCATAA	154
M-02716	Genomic	140	TGTCGTGCCTTGCAATAGTC	141	TGACAAGTCCTTCCTGGGTT	189
D7Cwr11D	Genomic	40	TTTGTCAAAATTCAATCTCCT	41	CAATCTTAGGGCAGAAATCA	85
D7Mlk1	Genomic	30	TGTGGCAGAGCAGACTGAAC	33	AGGAATTCTCCAAGAACTGCCAGACATG	345
D7Mlk2	Genomic	44	GTAGTGAGAACTTGTGGTTTCCA	45	TCGCTACTAAGGCCTAGCTTG	145
Picalm	cDNA	396	AAGGGGTGGCGGACGAG	146	CGCCAACTGTGGGATGTTC	205
Picalm	cDNA	147	CCAAGAAAAAGCACCTGGAC	148	TTTGAAGAGGAGCATGAAGG	495
Picalm	cDNA	149	CCAAAATCAAATGGATGCAC	150	TGCTTTTAAACGAGCCTGTTC	499
Picalm	cDNA	151	GGGAAAAACAGGCAGCATTAG	152	CAACATTAAGGCCAGCTGAAG	498, 348^†
Picalm	cDNA	153	CCTTCTCCAGTTGCACAGC	154	TGGTGGTCTCATGACAGG	498, 483, 474, 459^†
Picalm	cDNA	155	CCTGTAATGACACAACCAACC	156	TTTTATCCCCCAATGAATC	489
Picalm	Genomic	181	GCTGGAATTTTAAGGGAAAAG	182	GAATAAAGTTCTGTAGTTCACAAGG	388
Picalm	Genomic	214	CCCTACCCACCCACTAAGAAG	215	TGCCTGACAAAGACAGTCAATC	415

*Genomic, primers used for analysis of genomic DNA or BAC clone; cDNA, primers used in RT-PCR analysis.

^{†Sizes of the multiple RT-PCR products obtained from wild-type mice due to alternative splicing of the gene.}