Supplementary material for Sheppard et al. (2000) Proc. Natl. Acad. Sci. USA 97 (14), 7802-7807.

Fig. 6. Time course of spontaneous depurination of residue G17 within the 35mer DNA substrate. The reaction was performed under standard reaction conditions, employing 5'-³²P-labeled substrate in the absence of DNA enzyme (see Materials and Methods). The reaction products were either treated with piperidine (filled circles) or (open circles) before analysis by denaturing PAGE. Solid lines indicate a linear best fit to the data. Dashed line indicates the net amount of reaction that was attributable to depurination.