Supplementary material for Sheppard et al. (2000) Proc. Natl. Acad. Sci. USA 97 (14), 7802-7807.

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Fig. 7. Activity of the 10-28 N-glycosylase in the presence of various divalent metal cations. Self-cleavage reactions were performed in the presence of 2 mM divalent metal/10 mM NaCl/10 mM Mes (pH 5.2) at 25ºC. The metals are listed in order of the pKa values of the corresponding metal hydroxide.