Supplementary material for Sheppard et al. (2000) Proc. Natl. Acad. Sci. USA 97 (14), 7802-7807.
Fig. 8. Calcium dependence of the 10-28 N-glycosylase. Self-cleavage reactions were performed in the presence of 0.001-40 mM CaCl2/10 mM NaCl/50 mM Mes (pH 5.2) at 25ºC. Reaction products were treated with piperidine before analysis by denaturing PAGE. Two independent experiments were performed, employing either 3 or 4 nM DNA (filled and open circles, respectively).