Table 1.
OligonucleotidesLocus | Oligonucleotide* | Modification | Use† |
MID42 | 5'-tactatgtatttatacgttaagacctctatgaatgaatgta | 5' Dual biotin | Bound to beads |
MID42 | 5'-cgttaagacctctatgaatgaatgta | None | Forward primer for PCR |
MID42 | 5'-gaaaggtaagtacagggaaagg | None | Reverse primer for PCR |
MID42 | 5'-cacgcagattgaattaaacAGTTagttacaaagacacgtg | 5' 6-FAM | Hybridization probe for L allele |
MID42 | 5'-cacgcagattgaattaaacagttacaaagacacgtg | 5' Biotin | Hybridization probe for S allele |
calpain-10 | 5'-aggtcccagagggtggaaggagccaggacgcacccccactgctgctg | 5' Dual biotin | Bound to Beads |
calpain-10 | 5'-aggtcccagagggtggaag | None | Forward primer for PCR |
calpain-10 | 5'-ttgcgatggtcactgtgaag | None | Reverse primer for PCR |
calpain-10 | 5'-cacggtaggtgctTgcaggcagcgtg | 5' 6-FAM | Hybridization probe for A allele |
calpain-10 | 5'-cacggtaggtgccCgcaggcagcgtg | 5' Biotin | Hybridization probe for G allele |
KRAS2 | 5'-ttcgtccacaaaatgattctgaattagctgtatcgtcaagg | 5' Dual biotin | Bound to beads |
KRAS2 | 5'-agaatggtcctgcaccagtaa | None | Reverse primer for PCR |
KRAS2 | 5'-catgttctaatatagtcacattttca | None | Forward primer for PCR |
KRAS2 | 5'-cacgggagctGGTGGCgtagcgtg | 5' 6-FAM | Hybridization probe for wild-type allele |
KRAS2 | 5'-ccacgggagctgatggcgtagcgtgg | 5' Biotin | Hybridization probe for mutant allele |
6 FAM, 6-carboxyfluorescein.
*The bases in uppercase letters represent allelic differences.
†
Hybridization probes each contained 4 bases at their 5' and 3' ends to form hairpins, as explained in the text.