TIF1 Activates the Intra-S-Phase Checkpoint Response in the Diploid Micronucleus and Amitotic Polyploid Macronucleus of Tetrahymena -- Yakisich et al. 17 (12): 5185 Data Supplement - Supplemental Material -- Molecular Biology of the Cell

TIF1 Activates the Intra-S-Phase Checkpoint Response in the Diploid Micronucleus and Amitotic Polyploid Macronucleus of Tetrahymena
Mol. Biol. Cell Yakisich et al. 17: 5185

Supplemental Material

This article contains the following supporting material:

Supplemental Figure 1 - Wild type (CU428) and Tif1p-deficient strains (TXh48) were synchronized by starvation, released into fresh media, and pulse-labeled for 15 min with ³H-methyl-thymidine at 30 min intervals. Trichloroacetic acid precipitated material was subjected to scintillation counting. Data are representative of two independent experiments performed in quadruplicate.
Supplemental Figure 2 - Wild type (CU428) and tif1-1::neo/TIF1 mutant (TXh48) strains were grown to saturation, starved, and then released into drug-free media, (filled diamonds), media containing 20 mM HU (open triangles), or media containing 20 mM HU + 1 mM caffeine (filled squares, both drugs added at T= 0 min). Cell division was monitored by light microscopy, with the cell division index corresponding to the percentage of cells with a cytokinetic furrow.