Staphylococcus aureus Fibronectin Binding Protein-A Induces Motile Attachment Sites and Complex Actin Remodeling in Living Endothelial Cells -- Schröder et al. 17 (12): 5198 Data Supplement - Supplemental Material -- Molecular Biology of the Cell

Staphylococcus aureus Fibronectin Binding Protein-A Induces Motile Attachment Sites and Complex Actin Remodeling in Living Endothelial Cells
Mol. Biol. Cell Schröder et al. 17: 5198

Supplemental Material

This article contains the following supporting material:

Movie1 - Centripetal transport of FnBPA-S. carnosus clusters on the surface of an endothelial cell. Phase contrast movie of a cell section. (exposure time: 300 ms; frame rate: 15 f/s; sequence: 2232 s)
Movie2 - Centripetal transport of FnBPA-beads on the surface of an endothelial cell. Numbered and coloured lines mark tracks of selected beads. (exposure time: 400 ms; frame rate: 6 f/s; sequence: 3156 s)
Movie3 - Blocked motility of FnBPA-beads on an endothelial cell overexpressing GFP-tensin AH2. Numbered and coloured lines mark tracks of selected beads. (exposure time: 400 ms; frame rate: 6 f/s; sequence: 2223 s)
Movie4 - Multiple actin enrichments around FnBPA-S. carnosus clusters transported on the cell surface. Same cell and section as in movie-1 but showing GFP-actin fluorescence of a confocal time lapse series. (exposure time: 488 nm: 1000 ms; frame rate: 15 f/s; sequence 2341 s)
Movie5 - Actin wave advancing along a FnBPA-S. carnosus cluster. ROI (region of interest) from movie-5. (exposure time: 488 nm: 1000 ms; frame rate: 3 f/s; sequence 218 s)
Movie6a - Fast motion of a FnBPA-S. carnosus doublet on the endothelial cell surface. Phase contrast movie. (exposure time: 500 ms; frame rate: 10 f/s; sequence: 362 s)
Movie6b - FnBPA-S. carnosus doublet propelled by an actin comet tail on the endothelial cell surface. Same cell and section as in movie-3A but showing mRFP-actin fluorescence of confocal time lapse series. (exposure time: 568 nm: 1000 ms; frame rate: 10 f/s; sequence: 362 s)
Movie7 - Comet tail propelling a single FnBPA-S. carnosus bacterium towards the cell edge. GFP-Arp3 and mRFP-actin fluorescence (overlay) of a confocal time lapse series. (exposure time: 488: 1000 ms, 568: 1000 ms; frame rate: 5 f/s; sequence: 395 s)
Movie8 - Accumulation and colocalisation of actin and tensin at a FnBPA-S. carnosus tetrade. GFP-tensin and mRFP-actin fluorescence (overlay) of a confocal time lapse series. (exposure time: 488: 1000 ms, 568: 1000 ms; frame rate: 15 f/s; sequence: 698 s)
Movie9 - Alternating accumulation of actin and Rab5 at a FnBPA-S. carnosus tetrade. GFP-Rab5 and mRFP-actin fluorescence (overlay) of a confocal time lapse series. (exposure time: 488: 1000 ms, 568: 2000 ms; frame rate: 12 f/s; sequence: 1522 s)
Supplemental Figure 1 - Effect of tensin wild type and tensin AH2 on α5-integrin subunit localisation in endothelial cells. HUVEC were transfected with vectors expressing GFP (control), GFP-tensin wild type (wt) or GFP-tensin AH2 and then stained for α5-integrin subunit using a specific antibody (1:500; Millipore / Chemicon, Temecula, USA). The insets show the cells expressing GFP or GFP constructs. While tensin wild type increased the number of α5-containing fibrillary adhesions, tensin AH2 abolished them.