Supplementary material for Borkhardt et al., Proc. Natl. Acad. Sci. USA, 10.1073/pnas.150079597
Table 1. PCR primers used for amplification, cloning, sequencing, and GENESCAN analysis of Graf
Graf fragment | bp within Graf | Orientation | Sequence 5'® 3' | Size (bp) |
PCR primers |
|
|
|
|
C0 | 1-23 | sense, ext. + int. | GCACCATGGGGCTCCCAGCGCTC | 329 |
| 419-389 | antisense, ext. | TGTCTCTTTGTCATACTTCTTTTTGGCTTCC |
|
| 329-300 | antisense, int. | GGCATTCTCAATCATCCGTATCCGTTCATC |
|
|
|
|
|
|
C1 | 107-137 | sense, ext. + int. | ATTCATCAAGGAGCTCATCAAGGACGGGAAG | 603 |
| 755-727 | antisense, ext. | CATCAGTGATTCCACTTCTGATCTAGTGC |
|
| 709-683 | antisense, int. | GTGTTCTGTATGCTAATGGTTAACTGT |
|
|
|
|
|
|
C2 | 545-570 | sense, ext. | ATATGTCTTCAAGGTGCAGGAAGTCC | 836 |
| 580-609 | sense, int. | AGATGTTTGAGTTTGTGGAGCCCCTGCTGG |
|
| 1415-1388 | antisense, ext. + int. | TTGAAACTGGTACATCATGAGTGGTCCT |
|
|
|
|
|
|
C3 | 1257-1290 | sense, ext. | TCCAGAGTGCAGAAGTTGCTGAGTGTCCTGATGG | 447 |
| 1301-1332 | sense, int. | TGCTTCTGAGACAGAAACAGATATCTGTGCTG |
|
| 1747-1714 | antisense, ext. + int. | AGCTGGGCATTGGTGAGAGGCATATCGGGGCACGG |
|
|
|
|
|
|
C4 | 1632-1664 | sense, ext. | ACAGTAGCAGCCATCATGGACATCAAATTTCAG | 690 |
| 1666-1698 | sense, int. | ACATTGTCATTGAGATCCTAATAGAAAACCACG |
|
| 2354-2325 | antisense, ext. + int. | CCTCGACACTGGAATCAGCAGTTGTCATGG |
|
|
|
|
|
|
GENESCAN primers |
|
|
|
|
I FAM |
| sense, ext. | TGTGGAAACCAGAGGGATCAACGAG |
|
II |
| sense, int. | TGCTGAGTGTCCTGATGGTCATCG |
|
III |
| antisense, int. | CTCAGAAGCAGTCTTGGGGTCGTATG |
|
IV |
| antisense, ext. | CTGGTACATCATGAGTGGTCCTGG |
|
The last 6 bp of the 3' end of primers II and III are shown in bold and italics, indicating that these nucleotides correspond to the 52-bp insertion found in patient 7.