Supplementary material for Khodursky et al. (2000) Proc. Natl. Acad. Sci. USA 97 (17), 9419-9424.

Fig. 6. Effects of inactivation of DNA gyrase on plasmid supercoiling in vivo. Cultures of gyrB234 and gyrA43 strains containing plasmid pGP509 were shifted to 42ºC for the times indicated and the resulting topoisomers of pGP509 were visualized by gel electrophoresis. oc m, open circular monomer plasmid; sc d, supercoiled dimer; sc m, supercoiled monomer.