Fig. 3.

Localization of PARL Pβ domain in the matrix. (A) HeLa cells were transfected with a construct expressing PARL-HA-FCT (see scheme in C), fixed, and permeabilized with the indicated concentrations of digitonin. For each condition, cells were coimmunostained with anti-FLAG or anti-HA, and anti-Tom20 (to label the outer membrane), or anti-cytochrome c (to label the intermembrane space), or anti-peroxiredoxin 3 (to label the matrix). (B) Quantitation of the experiments shown in A. (C) Scheme summarizing the topology of PARL.