Colloquium Paper: Silencing leaf sorbitol synthesis alters long-distance partitioning and apple fruit quality -- Teo et al. 103 (49): 18842 Data Supplement - HTML Page - index.htslp -- Proceedings of the National Academy of Sciences

Teo et al. 10.1073/pnas.0605873103.

Supporting Information

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Supporting Figure 4
Supporting Table 6

Supporting Figure 4

Fig. 4. Gene expression of key proteins regulating sugar metabolism in control (open circles) and transgenic apple fruit, GSS68 (open diamonds), GSS04 (filled circles) and GSS27 (filled diamonds) at various stages of development, as revealed by real-time PCR. Each value is the mean (±SE) of six replicates.

Table 6. Stem water potential and carbon assimilation in leaves of control and transgenic lines

Time	8:20			10:15				12:30
Temperature, °C	27.5			31.3				34.6
	Stem water potential, MPa	Carbon assimilation, mmol·m^-2·s^-1		Stem water potential, MPa		Carbon assimilation, mmol·m^-2·s^-1		Stem water potential, MPa	Carbon assimilation, mmol·m^-2·s^-1
GSS68	-0.46 ± 0.03	19.06 ± 2.47		-0.74 ± 0.08		21.27 ± 2.09		-0.82 ± 0.01	17.47 ± 1.81
Control	-0.45 ± 0.03	19.32 ± 2.26		-0.74 ± 0.09		20.71 ± 2.50		-0.80 ± 0.09	17.80 ± 2.73
GSA27	-0.51 ± 0.03*	16.61. ± 1.19*		-0.71 ± 0.05		18.00 ± 2.63*		-0.80 ± 0.06	13.34 ± 1.42*
GSA04	-0.60 ± 0.06*	15.72 ± 1.87*		-0.87 ± 0.12*		16.20 ± 1.61*		-0.92 ± 0.07*	10.46 ± 3.80*
Time	14:20			16:30
Temp,°C	36.0			35.4
	Stem water potential, MPa		Carbon assimilation, mmol·m^-2·s^-1		Stem water potential, MPa		Carbon assimilation, mmol·m^-2·s^-1
GSS68	-0.78 ± 0.05		14.21 ± 4.21		-0.71 ± 0.05		11.89 ± 3.29
Control	-0.81 ± 0.12		16.38 ± 5.62		-0.74 ± 0.02		12.39 ± 2.82
GSA27	-0.73 ± 0.07		10.69 ± 1.76^*		-0.74 ± 0.04		11.28 ± 2.53
GSA04	-0.86 ± 0.08		8.09 ± 1.86^*		-0.87 ± 0.05		7.84 ± 0.98^*

Carbon assimilation rates and stomatal conductance of leaves were measured on grafted trees with a portable computerized open-system IRGA (LI-6400; LI-COR, Lincoln, NE) under ambient temperature and relative humidity. A cool light source (6400-02 LED) under software control was used. Carbon assimilation rates were measured on three different plants for each transgenic or control line. Three young, fully expanded leaves were measured per plant. Stem water potential was measured with a pressure chamber at midday 4 days after irrigation. Each value is the mean of three replicates ±SE.

*Significant difference from control at P ≤ 0.05.