Supplementary material for Incardona et al., Proc. Natl. Acad. Sci. USA, 10.1073/pnas.220251997

Fig. 7. ShhCD4 binding causes the retention of PtcHA at points of contact between cells. Shown are three different ShhCD4/PtcHA-expressing cell pairs. (Left) PtcHA immunofluorescence (green in the merged images); (Middle) Shh or CD4 immunofluorescence (red in the merged images); (Right) the merged images. The same cell pair is shown in C and D. The image in D represents a higher magnification of the region indicated by the white rectangle in C. C shows a focal plane within the cell interior, whereas D shows a focal plane at the cell surface. In addition to the large PtcHA⁺/Shh⁺ vesicles evident in C, the cell shows multiple small collections of PtcHA at points of contact, with fine dendritic processes of the ShhCD4-expressing cell seen in D.