Supplementary material for Cho et al., Proc. Natl. Acad. Sci. USA, 10.1073/pnas.210391497
Table 1. Synthetic MTB-derived peptides with high HLA-A*0201-binding affinities
(IC50 £ 50 nM)
Amino acid sequence | Source | Amino acid position | A*0201 nM |
FLMPVEDVFI | Elongation factor Tu | 212-221 | 3.3 |
AMALLRLPLV | Thymidylate synthase (EC 2.1.1.45) | 25-34 | 5.1 |
RLPLVLPAV | Thymidylate synthase (EC 2.1.1.45) | 30-38 | 5.1 |
YLYVHSPAL | Virulence-regulating protein | 104-112 | 5.4 |
ILFTFLHLA | Alanine dehydrogenase | 92-100 | 5.4 |
YLDLALMSV | Mycocerosic acid synthase (MAS) | 1,679-1,687 | 5.5 |
ALVLLMLPVV | PstA1 | 269-278 | 6.8 |
FVVALIPLV | PstA1 | 145-153 | 7.6 |
GLMTAVYLV | PstA1 | 211-219 | 8.9 |
YLLALRYLA | 3-dehydroquinase | 134-142 | 10.0 |
SLYFGGICV | PstA1 | 75-83 | 10.6 |
YLSQIAVLL | Virulence-regulating protein | 299-307 | 12.1 |
MTYAAPLFV | RpoB gene | 127-135 | 13.8 |
LLLDVTPLSL | 70k | 364-373 | 17.0 |
GLYYLTTEV | RpoC gene | 71-79 | 20.8 |
LLGSFELTGI | 70k | 427-436 | 26.0 |
YTYKWETFL | 85c | 137-145 | 29.0 |
AMPAYNWMTV | inhA | 154-163 | 27.7 |
GLFLTTEAV | 65k | 509-517 | 31.0 |
ALVLLMLPV | PstA1 | 269-277 | 31.2 |
LLFRFMRPL | Gyrase B gene | 558-566 | 35.7 |
RMFAANLGV | Thymidylate synthase (EC 2.1.1.45) | 386-394 | 35.7 |
LLWQDPVPA | Catalase (EC 1.11.1.6) HPI | 435-443 | 45.4 |
ILSSLGLPV | 3-dehydroquinate synthase (EC 4.6.1.3) | 297-305 | 50.0 |
LLFLGVVFL | AT103 protein | 27-35 | 50.0 |
VLLLDVTPL | 70k | 363-371 | 50.0 |
SLLEIGEGVV | 70k | 179-188 | 50.0 |