Supplementary material for Cho et al., Proc. Natl. Acad. Sci. USA, 10.1073/pnas.210391497
Table 2. Synthetic MTB-derived peptides with intermediate HLA-A*0201-binding affinities (50 nM < IC50 £ 500 nM)
Amino acid sequence | Source | Amino acid position | A*0201 nM |
MALLRLPLV | Thymidylate synthase (EC 2.1.1.45) | 26-34 | 52.6 |
LMIGTAAAVV | 85B | 15-24 | 54.0 |
RMSRVTTFTV | PstA1 | 225-234 | 75.5 |
TVLRFVPPL | aroA gene | 97-108 | 76.9 |
LMIGTAAAV | 85B | 15-23 | 79.0 |
LLFRFMRPLI | Gyrase B gene | 558-567 | 83.3 |
GLGGLGLFFA | Mycocerosic acid synthase (MAS) | 1,771-1,780 | 89.2 |
ALLSDWLPA | inhA | 244-252 | 94.3 |
RLLDDTPEV | Tuberculin-active protein | 2-10 | 96.1 |
GLYLSQIAV | Virulence-regulating protein | 297-305 | 96.1 |
AMLQDMAIL | 65k | 285-293 | 98.0 |
SLLEIGEGV | 70k | 179-187 | 98.0 |
AVHDTLFYCV | Alanine dehydrogenase | 291-300 | 104.0 |
FLTCDANLAV | MAS | 1,515-1,524 | 104.1 |
LLLETSWEAI | MAS | 97-106 | 106.3 |
MLATDLSLRV | Catalase (EC 1.11.1.6) HPI | 376-385 | 108.6 |
GLAGTVLRFV | aroA gene | 93-102 | 108.6 |
SLAGFVRML | Virulence-regulating protein | 48-57 | 111.1 |
VLIAFGRFPI | MAS | 1,462-1,471 | 113.6 |
FLLADERVRV | Biotin carboxyl carrier protein | 426-435 | 116.2 |
FMSLAGFVRM | Virulence-regulating protein | 46-55 | 116.2 |
RMPAVTDLV | 70k | 318-326 | 125.0 |
DMWEHAFYL | Superoxide dismutase | 160-168 | 132.0 |
LLCCSGVATA | Antigen MPB64/MPT64 | 14-23 | 151.5 |
GIVSGILLSI | PstA1 | 319-328 | 151.5 |
RLVSGLVGA | 32k | 25-33 | 152.0 |
ATYDKGILTV | 14 kDa antigen | 115-124 | 156.2 |
SLYFGGICVI | PstA1 | 75-84 | 166.6 |
AMKADIQHV | 85c | 317-325 | 167.0 |
MLQDMAILT | 65k | 286-294 | 167.0 |
RLQPQVGWEV | Catalase (EC 1.11.1.6) HPI | 496-505 | 178.5 |
GLYRQWALA | Biotin carboxyl carrier protein | 464-473 | 185.1 |
KLTADGVLTA | MAS | 1,702-1,711 | 200.0 |
LLAAGVADPV | 65k | 485-494 | 217.0 |
GLVDFVKHI | Gyrase B gene | 267-276 | 217.3 |
RLMIGTAAA | 85B | 14-22 | 227.0 |
MLGNAPSVV | MTp40 protein | 1-9 | 227.2 |
LMLPGMNGI | H37Rv MtrA gene | 54-62 | 238.0 |
GLFLTTEAVV | 65k | 509-518 | 238.0 |
GLYGAQYDV | Diaminopimelate decarboxylase (EC 4.1.1.20) | 346-354 | 250.0 |
MLASTLTDA | MAS | 1,224-1,233 | 250.0 |
LLACAVIHA | Transposase | 289-297 | 250.0 |
LLATVFKLTA | MAS | 1,696-1,705 | 250.0 |
ALYLLDGLRA | 32k | 77-86 | 263.0 |
VLLEDGGVEV | Gyrase B gene | 79-88 | 277.7 |
YLSEGDMAA | Protein antigen b (Pab) gene | 108-116 | 294.1 |
WMAETTLGRV | rpoC gene | 149-158 | 294.1 |
AIAWGAWGEV | MAS | 1,934-1,943 | 312.5 |
AIYHPQQFV | 32k | 178-186 | 313.0 |
VLLEDGGVEV | Gyrase B gene | 130-139 | 333.3 |
LLPLGYPFV | rpoC gene | 163-172 | 333.3 |
LALVLLMLPV | PstA1 | 265-277 | 333.3 |
KLAGGVAVI | 65k | 369-377 | 357.0 |
WLLIDTSNA | MAS | 1,210-1,219 | 357.1 |
FLLPDAQSI | Pab gene | 273-281 | 384.6 |
RIANGMGATV | Alanine dehydrogenase | 187-196 | 385.0 |
KIFMLVTAVV | Antigen MPB64/MPT64 | 4-13 | 416.6 |
ILLSIARVV | PstA1 | 324-332 | 454.5 |