Li et al. 10.1073/pnas.0607245103. |
Fig. 7. (A) Tm salt dependence of I-motif (1 mM in strand) in the absence (squares) or presence (circles) of SWNTs (10 mg·ml-1) in aqueous cacodylic buffer (0.1 mM cacodylic acid/sodium cacodylate, pH 5.5) with 50 mM, 100 mM, 150 mM, and 200 mM NaCl. (B) CD spectra of I-motif (1 mM in strand) in the absence (solid line) or presence (open circles) of SWNTs (10 mg·ml-1) in aqueous cacodylic buffer (0.1 mM cacodylic acid/sodium cacodylate/100 mM NaCl, pH 5.5). (C) CD spectra of G-quadruplex (1 mM in strand) in the absence (solid line) or presence (open circles) of SWNTs (10 mg·ml-1) in aqueous cacodylic buffer (0.1 mM cacodylic acid/sodium cacodylate/100 mM NaCl, pH 7.0).
Fig. 8. Plots of fluorescence emission intensity of 5'-fluorescein and 3'-tetramethylrhodamine (TAMRA)-labeled C-rich strand at 520 nm versus the concentration of its complementary G-rich strand in 0.1 mM cacodylic acid/sodium cacodylate and 100 mM NaCl at pH 7.0 in the absence of SWNTs (solid squares) or the presence of 10 mg·ml-1 SWNTs (open squares) and at pH 5.0 in the absence of SWNTs (solid circles) or the presence of 10 mg·ml-1 SWNTs (open circles).
Table 1. Thermodynamic parameters of d(CCCTAA)3CCCT, d(AGGGTT)3AGGG and equimolar mixture of d(CCCTAA)3CCCT/d(AGGGTT)3AGGG in aqueous cacodylic buffer (0.1 mM cacodylic acid/sodium cacodylate/100 mM NaCl)
DNA | pH | Δ H (kJ·mol-1) | Δ S (J·mol-1·K-1) | Δ G20 (kJ·mol-1) | T m (°C) |
C-rich strand | 5.5 | -207.6 ± 2.9 | -668.2 ± 9.3 | -11.8 ± 0.2 | 38 |
SWNTs- C-rich stranda | 5.5 | -180.9 ± 1.9 | -544.9 ± 5.6 | -21.2 ± 0.3 | 60 |
7.0 | -192.0 ± 2.7 | -615.4 ± 8.5 | -11.7 ± 0.2 | 38 | |
8.0 | -204.7 ± 2.2 | -660.0 ± 7.0 | -11.3 ± 0.1 | 38 | |
G-rich strand | 5.5 | -143.4 ± 2.0 | -431.0 ± 6.3 | -17.1 ± 0.2 | 61 |
7.0 | -200.4 ± 0.8 | -602.8 ± 2.3 | -23.8 ± 0.1 | 60 | |
SWNTs- G-rich stranda | 5.5 | -148.0 ± 1.1 | -444.3 ± 3.4 | -17.8 ± 0.1 | 61 |
7.0 | -180.8 ± 1.1 | -545.0 ± 3.2 | -21.1 ± 0.2 | 60 | |
WC-duplexb | 5.5 | -508.4 ± 4.7 | -1376.8 ± 13.9 | -105.0 ± 0.6 | 67 |
7.0 | -484.8 ± 6.1 | -1309.7 ± 18.0 | -101.1 ± 0.8 | 67 | |
SWNTs-WC-duplexa,b | 5.5 | -557.1 ± 5.2 | -1531.4 ± 15.5 | -108.4 ± 0.7 | 65 |
7.0 | -515.1 ± 4.6 | -1403.8 ± 13.8 | -103.8 ± 0.6 | 65 |
a
SWNTs: 10 μg·ml-1.b
Duplex: 2 μM in strand.