Li et al. 10.1073/pnas.0607245103.

Supporting Information

Files in this Data Supplement:

Supporting Figure 7
Supporting Table 1
Supporting Figure 8




Supporting Figure 7

Fig. 7. (A) Tm salt dependence of I-motif (1 mM in strand) in the absence (squares) or presence (circles) of SWNTs (10 mg·ml-1) in aqueous cacodylic buffer (0.1 mM cacodylic acid/sodium cacodylate, pH 5.5) with 50 mM, 100 mM, 150 mM, and 200 mM NaCl. (B) CD spectra of I-motif (1 mM in strand) in the absence (solid line) or presence (open circles) of SWNTs (10 mg·ml-1) in aqueous cacodylic buffer (0.1 mM cacodylic acid/sodium cacodylate/100 mM NaCl, pH 5.5). (C) CD spectra of G-quadruplex (1 mM in strand) in the absence (solid line) or presence (open circles) of SWNTs (10 mg·ml-1) in aqueous cacodylic buffer (0.1 mM cacodylic acid/sodium cacodylate/100 mM NaCl, pH 7.0).





Supporting Figure 8

Fig. 8. Plots of fluorescence emission intensity of 5'-fluorescein and 3'-tetramethylrhodamine (TAMRA)-labeled C-rich strand at 520 nm versus the concentration of its complementary G-rich strand in 0.1 mM cacodylic acid/sodium cacodylate and 100 mM NaCl at pH 7.0 in the absence of SWNTs (solid squares) or the presence of 10 mg·ml-1 SWNTs (open squares) and at pH 5.0 in the absence of SWNTs (solid circles) or the presence of 10 mg·ml-1 SWNTs (open circles).





Table 1. Thermodynamic parameters of d(CCCTAA)3CCCT, d(AGGGTT)3AGGG and equimolar mixture of d(CCCTAA)3CCCT/d(AGGGTT)3AGGG in aqueous cacodylic buffer (0.1 mM cacodylic acid/sodium cacodylate/100 mM NaCl)

DNA

pH

Δ

H (kJ·mol-1)

Δ

S (J·mol-1·K-1)

Δ

G20 (kJ·mol-1)

T

m (°C)

C-rich strand

5.5

-207.6 ± 2.9

-668.2 ± 9.3

-11.8 ± 0.2

38

SWNTs- C-rich stranda

5.5

-180.9 ± 1.9

-544.9 ± 5.6

-21.2 ± 0.3

60

7.0

-192.0 ± 2.7

-615.4 ± 8.5

-11.7 ± 0.2

38

8.0

-204.7 ± 2.2

-660.0 ± 7.0

-11.3 ± 0.1

38

G-rich strand

5.5

-143.4 ± 2.0

-431.0 ± 6.3

-17.1 ± 0.2

61

7.0

-200.4 ± 0.8

-602.8 ± 2.3

-23.8 ± 0.1

60

SWNTs- G-rich stranda

5.5

-148.0 ± 1.1

-444.3 ± 3.4

-17.8 ± 0.1

61

7.0

-180.8 ± 1.1

-545.0 ± 3.2

-21.1 ± 0.2

60

WC-duplexb

5.5

-508.4 ± 4.7

-1376.8 ± 13.9

-105.0 ± 0.6

67

7.0

-484.8 ± 6.1

-1309.7 ± 18.0

-101.1 ± 0.8

67

SWNTs-WC-duplexa,b

5.5

-557.1 ± 5.2

-1531.4 ± 15.5

-108.4 ± 0.7

65

7.0

-515.1 ± 4.6

-1403.8 ± 13.8

-103.8 ± 0.6

65

a

SWNTs: 10 μg·ml-1.

b

Duplex: 2 μM in strand.