Supplementary material for Lohr and Wilhelm (1999) Proc. Natl. Acad. Sci. USA 96(15), 8784-8789.

Table 3.

 Chromatographic, spectral, and mass spectrometric properties of the xanthophyll-cycle pigments identified in Phaeodactylum tricornutum

Pigment

Rt, min

Rf (TLC)

Absorption maxima, nm

III / II ratio

Expected mol. wt.

Main peak

(MALDI)

Ion

species

Vx

13.0

0.52

Gradient

418

442

472

0.98

600.4

599.4

[M-1]+

Vx

13.0

0.52

EtOH

418 (417)

441

471

0.93 (0.97)

 

(599.3)

 

Vx

13.0

0.52

EtOH + HCl

381

402

427

0.96 (0.97)

 

 

 

Ddx

14.4

0.60

Gradient

-

449

479

0.73

582.4

581.5

[M-1]+

Ax

15.2

0.64

Gradient

-

449

476

0.64

584.4

583.6

[M-1]+

Ax

15.2

0.64

EtOH

425

446

475 (474)

0.60 (0.50)

 

(583.5)

 

Ax

15.2

0.64

EtOH + HCl

407 (405)

427 (426)

453 (452)

0.41 (0.40)

 

 

 

Dtx

16.3

0.72

Gradient

-

455

484

0.43

566.4

566.5

[M]+

Zx

17.1

0.75

Gradient

-

455

482

0.31

568.4

568.4

[M]+

Zx

17.1

0.75

EtOH

-

451

479

0.28 (0.33)

 

 

 

Zx

17.1

0.75

EtOH + HCl

-

450 (449)

475 (474)

0.11 (0.05)

 

 

 

The corresponding data of the reference pigments Vx, Ax, and Zx isolated from spinach are denoted (in italics) only when differing from those of the pigments in P. tricornutum. Rt, HPLC-retention time; mol. wt., molecular weight; MALDI, matrix-assisted laser desorption ionization/time-of-flight-MS; Ax, antheraxanthin; Ddx, diadinoxanthin; Dtx, diatoxanthin; Vx, violaxanthin; Zx, zeaxanthin.