Jiang et al. 10.1073/pnas.0606775103. |
Fig. 5. Reversed-phase HPLC purification of histone classes. (Upper) HPLC elution profile for calf thymus histones, as described in Materials and Methods; numbers above peaks represent fraction labels used as column headings in Lower. (Lower) SDS/PAGE analysis of proteins in HPLC fractions noted Upper.
Fig. 6. Reversed-phase HPLC resolution (with MS/MS detection) of PITC derivatives of N6-formyl-lysine and arginine. (Upper) Standard PITC derivatives of N6-formyl-lysine and arginine. (Lower) HPLC fraction containing the PITC derivative of N6-formyl-lysine derived from histone proteins.
Fig. 7. Examples of calibration curves for the isotope dilution LC-MS/MS analysis of PITC derivatives of N6-formyl-lysine and lysine. (Upper) Calibration curve for N6-formyl-lysine; FK, N6-formyl-lysine; DFK, deuterium-labeled N6-formyl-lysine. (Lower) Calibration curve for lysine; K, lysine; DK, deuterium-labeled lysine.
Fig. 8. ESI-TOF high-resolution mass spectrometric analysis of PITC derivatives of N6-formyl-lysine and arginine. (Upper) Standard PITC derivative of N6-formyl-lysine. (Lower) HPLC fraction containing the PITC derivative of N6-formyl-lysine derived from histone proteins.